Detailed information of TA system TA225240

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 101485..102020
• Replicon: plasmid p16_1068_1
• Accession: NZ_CP087288
• Organism: Escherichia coli strain APEC 16-1068

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0VJJ7
• Locus tag: LO742_RS25630
• Protein ID: WP_000222760.1
• Coordinates: 101485..101772 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0VIP2
• Locus tag: LO742_RS25635
• Protein ID: WP_001132900.1
• Coordinates: 101769..102020 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO742_RS25600 (97068)	97068..98289	-	1222	Protein_94	ISL3 family transposase	-
LO742_RS25605 (98389)	98389..98753	+	365	Protein_95	IS1 family transposase	-
LO742_RS25610 (98808)	98808..99590	-	783	WP_001442137.1	IS21-like element IS100kyp family helper ATPase IstB	-
LO742_RS25615 (99587)	99587..100609	-	1023	WP_000255956.1	IS21-like element IS100 family transposase	-
LO742_RS25620 (100698)	100698..101045	+	348	Protein_98	IS1-like element IS1A family transposase	-
LO742_RS25625 (101061)	101061..101381	-	321	Protein_99	serine acetyltransferase	-
LO742_RS25630 (101485)	101485..101772	-	288	WP_000222760.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LO742_RS25635 (101769)	101769..102020	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LO742_RS25640 (102915)	102915..105881	-	2967	WP_001138014.1	Tn3-like element TnAs1 family transposase	-
LO742_RS25645 (105885)	105885..106445	-	561	WP_001161490.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..195721	195721
	-	inside	IScluster/Tn	-	-	97330..106445	9115

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11105.15 Da
• Isoelectric Point: 10.5832

>T225240 WP_000222760.1 NZ_CP087288:c101772-101485 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Nucleotide

• Length: 288 bp

>T225240 NZ_CP121077:14789-15121 [Salmonella enterica subsp. enterica serovar Infantis]
ATGGACAGAGGGGAAATCTGGCTCGTCTCACTGGATCCTACAGCCGGACATGAACAAAGCGGAAAACGTCCGGTACTTAT
CGTTTCTCCGGCATCATTTAATAAGTTCACCCGGTTACCCGTTGTCGTTCCCGTCACCAGCGGCGGAGACTTTGCCCGCA
CAGCCGGTTTTACCGTCTCCCTGGACGGCGCGGGAACAAAAACAACGGGTGTTATTCGCTGCGACCAGCCCAGAACGATT
GATATGGGTGCCCGGAATGGTAAGCGTCTGGAACGTATACCCGAAGCTGTCGTAAATGAAGTGCTGGCCCGACTGGAAGC
CATTCTGAGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9211.41 Da
• Isoelectric Point: 4.0780

>AT225240 WP_001132900.1 NZ_CP087288:c102020-101769 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT225240 NZ_CP121077:14531-14788 [Salmonella enterica subsp. enterica serovar Infantis]
ATGTATACCACTCGTCTGAAAAAGGTTGGCGGATCCGTCATGCTGGCGGTTCCTCCCGCCGTGTTGAAAACGCTGGCTCT
GTCGACAGACAGCGAAGTGGGAATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAACTGCTGGCACAGTGCGATCCGCACGCCGAAATGAGCGATGAGGATAGGGAATGGATGGATGCGCCTGCAGTG
GGTAAGGAGATCCTGTAA

Structures

Toxin

• AlphaFold DB: V0VJJ7

Antitoxin

• AlphaFold DB: V0VIP2