Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3887789..3888443 Replicon chromosome
Accession NZ_CP087287
Organism Escherichia coli strain APEC 16-1068

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag LO742_RS19265 Protein ID WP_000244781.1
Coordinates 3887789..3888196 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LO742_RS19270 Protein ID WP_000354046.1
Coordinates 3888177..3888443 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LO742_RS19245 (3883746) 3883746..3885479 - 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -
LO742_RS19250 (3885485) 3885485..3886195 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LO742_RS19255 (3886220) 3886220..3887116 - 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
LO742_RS19260 (3887228) 3887228..3887749 + 522 WP_001055874.1 flavodoxin FldB -
LO742_RS19265 (3887789) 3887789..3888196 - 408 WP_000244781.1 protein YgfX Toxin
LO742_RS19270 (3888177) 3888177..3888443 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LO742_RS19275 (3888686) 3888686..3889666 + 981 WP_000886078.1 tRNA-modifying protein YgfZ -
LO742_RS19280 (3889743) 3889743..3890402 - 660 WP_000250274.1 hemolysin III family protein -
LO742_RS19285 (3890566) 3890566..3890877 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
LO742_RS19290 (3890922) 3890922..3892355 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T225237 WP_000244781.1 NZ_CP087287:c3888196-3887789 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T225237 NZ_CP121076:c4183007-4182723 [Salmonella enterica subsp. enterica serovar Infantis]
ATGAATTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT225237 WP_000354046.1 NZ_CP087287:c3888443-3888177 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT225237 NZ_CP121076:c4183239-4182997 [Salmonella enterica subsp. enterica serovar Infantis]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCCTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGAATTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References