Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3733908..3734491 Replicon chromosome
Accession NZ_CP087287
Organism Escherichia coli strain APEC 16-1068

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag LO742_RS18635 Protein ID WP_000254750.1
Coordinates 3733908..3734243 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag LO742_RS18640 Protein ID WP_000581937.1
Coordinates 3734243..3734491 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LO742_RS18620 (3729794) 3729794..3731092 - 1299 WP_000036723.1 phosphopyruvate hydratase -
LO742_RS18625 (3731180) 3731180..3732817 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
LO742_RS18630 (3733045) 3733045..3733836 - 792 WP_001071651.1 nucleoside triphosphate pyrophosphohydrolase -
LO742_RS18635 (3733908) 3733908..3734243 - 336 WP_000254750.1 endoribonuclease MazF Toxin
LO742_RS18640 (3734243) 3734243..3734491 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
LO742_RS18645 (3734569) 3734569..3736803 - 2235 WP_000226815.1 GTP pyrophosphokinase -
LO742_RS18650 (3736851) 3736851..3738152 - 1302 WP_274429255.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T225236 WP_000254750.1 NZ_CP087287:c3734243-3733908 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T225236 NZ_CP121076:3717303-3717560 [Salmonella enterica subsp. enterica serovar Infantis]
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCGAAACCTGCATGACCACCAGTTACTCACATATCCCCAGCCA
GCACCTTAAGGGTGACTGGCAGGAAGAAGCGGGATTTGAGACCGGGCGCAGCGTGACCGTGAAGATTTCACAGGGGTGTA
TTGTGCTGATGGCGGACAGTAACGAGGAGCAGAAGCTGCGCGAACAGCTTTACAAGGCTGAACAGGTGGTTAAGGGGATG
CGGGACGTGATTGTTTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT225236 WP_000581937.1 NZ_CP087287:c3734491-3734243 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT225236 NZ_CP121076:c3717478-3717146 [Salmonella enterica subsp. enterica serovar Infantis]
TCCGCCATCAGCACAATACACCCCTGTGAAATCTTCACGGTCACGCTGCGCCCGGTCTCAAATCCCGCTTCTTCCTGCCA
GTCACCCTTAAGGTGCTGGCTGGGGATATGTGAGTAACTGGTGGTCATGCAGGTTTCGCTGTTGATGTGGCGGACGCTGA
CGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTCTCCGCCGGATGCTGGCTGATTGTGGAGCTCAGGGTG
AGCGAGTATGGGCGCGACATCCTGGCACCGCGCCTCCCTCTCCTCGGCCAGCACCGGGCGGTGATGAGCAACCGGCTACC
GGGGCCGTATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References