Detailed information of TA system TA225226

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2327400..2328988
• Replicon: chromosome
• Accession: NZ_CP087287
• Organism: Escherichia coli strain APEC 16-1068

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B7MMY1
• Locus tag: LO742_RS11550
• Protein ID: WP_001125475.1
• Coordinates: 2327400..2328722 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LO742_RS11555
• Protein ID: WP_001332145.1
• Coordinates: 2328722..2328988 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO742_RS11530 (2322747)	2322747..2325398	-	2652	WP_001350647.1	fimbria/pilus outer membrane usher protein	-
LO742_RS11535 (2325440)	2325440..2326150	-	711	WP_001195181.1	fimbria/pilus periplasmic chaperone	-
LO742_RS11540 (2326157)	2326157..2326774	-	618	Protein_2251	SDR family oxidoreductase	-
LO742_RS11545 (2326908)	2326908..2327090	+	183	Protein_2252	hypothetical protein	-
LO742_RS11550 (2327400)	2327400..2328722	-	1323	WP_001125475.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
LO742_RS11555 (2328722)	2328722..2328988	-	267	WP_001332145.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LO742_RS11560 (2329296)	2329296..2330054	+	759	WP_001286588.1	trans-aconitate 2-methyltransferase	-
LO742_RS11565 (2330058)	2330058..2330972	-	915	WP_001306069.1	bestrophin family protein	-
LO742_RS11570 (2331169)	2331169..2332620	-	1452	WP_000854637.1	tagaturonate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2313521..2328988	15467

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49426.24 Da
• Isoelectric Point: 8.9380

>T225226 WP_001125475.1 NZ_CP087287:c2328722-2327400 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRRNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVMCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T225226 NZ_CP121075:c4343756-4343472 [Salmonella enterica subsp. enterica serovar Infantis]
ATGAATTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.44 Da
• Isoelectric Point: 6.2267

>AT225226 WP_001332145.1 NZ_CP087287:c2328988-2328722 [Escherichia coli]
MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPNNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT225226 NZ_CP121075:c4343988-4343746 [Salmonella enterica subsp. enterica serovar Infantis]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCCTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGAATTA
TGA

Structures

Toxin

• AlphaFold DB: A0A0E2L5M8