Detailed information of TA system TA225212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 125782..126425
• Replicon: plasmid pYUSHP29-2
• Accession: NZ_CP087282
• Organism: Leclercia adecarboxylata strain SH19PE29

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: LO739_RS24050
• Protein ID: WP_230431572.1
• Coordinates: 125782..126198 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: LO739_RS24055
• Protein ID: WP_230431573.1
• Coordinates: 126195..126425 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO739_RS24030 (LO739_24025)	120923..121942	-	1020	WP_230431570.1	alpha/beta hydrolase	-
LO739_RS24035 (LO739_24030)	122641..123063	+	423	WP_117089457.1	MarR family transcriptional regulator	-
LO739_RS24040 (LO739_24035)	123459..124931	+	1473	WP_230431571.1	aldehyde dehydrogenase family protein	-
LO739_RS24045 (LO739_24040)	125026..125652	+	627	WP_230431594.1	TetR/AcrR family transcriptional regulator	-
LO739_RS24050 (LO739_24045)	125782..126198	-	417	WP_230431572.1	type II toxin-antitoxin system VapC family toxin	Toxin
LO739_RS24055 (LO739_24050)	126195..126425	-	231	WP_230431573.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LO739_RS24060 (LO739_24055)	126538..126789	+	252	WP_230431574.1	hypothetical protein	-
LO739_RS24065 (LO739_24060)	126909..127484	-	576	WP_230431575.1	SLATT domain-containing protein	-
LO739_RS24070 (LO739_24065)	127492..128820	-	1329	WP_230431576.1	nucleotidyltransferase	-
LO739_RS24075 (LO739_24070)	129010..130377	-	1368	WP_230431577.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..132089	132089

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15186.48 Da
• Isoelectric Point: 5.7444

>T225212 WP_230431572.1 NZ_CP087282:c126198-125782 [Leclercia adecarboxylata]
VNKTYMLDTNICSFIMREQPEAVLKRLEQAVLRNQRIVVSAITYSEMRFGATGPKASPRHTELVDEFCARLDAILPWDRA
AVDASTDVKVALRLAGTPIGPNDTLIAGHAIAAGAILVTNNVREFERVPGLVLEDWVN

Nucleotide

• Length: 417 bp

>T225212 NZ_CP121074:14789-15121 [Salmonella enterica subsp. enterica serovar Infantis]
ATGGACAGAGGGGAAATCTGGCTCGTCTCACTGGATCCTACAGCCGGACATGAACAAAGCGGAAAACGTCCGGTACTTAT
CGTTTCTCCGGCATCATTTAATAAGTTCACCCGGTTACCCGTTGTCGTTCCCGTCACCAGCGGCGGAGACTTTGCCCGCA
CAGCCGGTTTTACCGTCTCCCTGGACGGCGCGGGAACAAAAACAACGGGTGTTATTCGCTGCGACCAGCCCAGAACGATT
GATATGGGTGCCCGGAATGGTAAGCGTCTGGAACGTATACCCGAAGCTGTCGTAAATGAAGTGCTGGCCCGACTGGAAGC
CATTCTGAGCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8688.80 Da
• Isoelectric Point: 4.4692

>AT225212 WP_230431573.1 NZ_CP087282:c126425-126195 [Leclercia adecarboxylata]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVTNEGRVDL

Nucleotide

• Length: 231 bp

>AT225212 NZ_CP121074:14531-14788 [Salmonella enterica subsp. enterica serovar Infantis]
ATGTATACCACTCGTCTGAAAAAGGTTGGCGGATCCGTCATGCTGGCGGTTCCTCCCGCCGTGTTGAAAACGCTGGCTCT
GTCGACAGACAGCGAAGTGGGAATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAACTGCTGGCACAGTGCGATCCGCACGCCGAAATGAGCGATGAGGATAGGGAATGGATGGATGCGCCTGCAGTG
GGTAAGGAGATCCTGTAA