Detailed information of TA system TA225211

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 75583..76322
• Replicon: plasmid pYUSHP29-2
• Accession: NZ_CP087282
• Organism: Leclercia adecarboxylata strain SH19PE29

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: LO739_RS23810
• Protein ID: WP_172693814.1
• Coordinates: 75583..76068 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: LO739_RS23815
• Protein ID: WP_172693815.1
• Coordinates: 76056..76322 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO739_RS23780 (LO739_23775)	71268..71579	+	312	WP_045898923.1	type II toxin-antitoxin system HigB family toxin	-
LO739_RS23785 (LO739_23780)	71576..71995	+	420	WP_102752378.1	helix-turn-helix domain-containing protein	-
LO739_RS23790 (LO739_23785)	72158..73305	+	1148	WP_230431009.1	IS3 family transposase	-
LO739_RS23795 (LO739_23790)	73372..73683	+	312	WP_230431557.1	fimbrial protein	-
LO739_RS23800 (LO739_23795)	73830..74949	-	1120	Protein_80	IS3-like element ISKpn34 family transposase	-
LO739_RS23805 (LO739_23800)	75120..75245	-	126	WP_230431592.1	hypothetical protein	-
LO739_RS23810 (LO739_23805)	75583..76068	-	486	WP_172693814.1	GNAT family N-acetyltransferase	Toxin
LO739_RS23815 (LO739_23810)	76056..76322	-	267	WP_172693815.1	DUF1778 domain-containing protein	Antitoxin
LO739_RS23820 (LO739_23815)	76966..77979	+	1014	WP_172693743.1	plasmid replication initiator RepA	-
LO739_RS23825 (LO739_23820)	78758..79519	-	762	WP_230431593.1	TraX family protein	-
LO739_RS23830 (LO739_23825)	80269..80598	-	330	WP_230431558.1	hypothetical protein	-
LO739_RS23835 (LO739_23830)	80595..81236	-	642	WP_230431559.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..132089	132089
	-	inside	IScluster/Tn	-	-	62065..74949	12884

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (43-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17519.25 Da
• Isoelectric Point: 10.1390

>T225211 WP_172693814.1 NZ_CP087282:c76068-75583 [Leclercia adecarboxylata]
VGRVTAPEPLSNSHQLAEFVSGEAVLDDWLKQRGLKNQALGAARTFVVCKTGTKLVAGFYSLATGSVNHVEATGSLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVLRCYRVADNIGVRAIMVHALTEQAKSFYLHHGFKASQTQERTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T225211 NZ_CP121073:4661364-4661675 [Salmonella enterica subsp. enterica serovar Infantis]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATACTATCGGGGTAATAAAGAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10048.46 Da
• Isoelectric Point: 4.6027

>AT225211 WP_172693815.1 NZ_CP087282:c76322-76056 [Leclercia adecarboxylata]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVANDAAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT225211 NZ_CP121073:4661672-4662118 [Salmonella enterica subsp. enterica serovar Infantis]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTCAAACTACCAGCAGATGCCTTCATCGAGTAG