225209

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 70478..71121 Replicon plasmid pYUSHP29-1
Accession NZ_CP087281
Organism Leclercia adecarboxylata strain SH19PE29

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag LO739_RS23355 Protein ID WP_000754566.1
Coordinates 70478..70894 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag LO739_RS23360 Protein ID WP_001261276.1
Coordinates 70891..71121 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LO739_RS23335 (LO739_23330) 67336..67419 - 84 Protein_77 SOS response-associated peptidase -
LO739_RS23340 (LO739_23335) 67939..68040 + 102 Protein_78 Tn3 family transposase -
LO739_RS23345 (LO739_23340) 68060..69136 - 1077 WP_000227969.1 IS110 family transposase -
LO739_RS23350 (LO739_23345) 69418..70274 - 857 Protein_80 IS3-like element ISEc15 family transposase -
LO739_RS23355 (LO739_23350) 70478..70894 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
LO739_RS23360 (LO739_23355) 70891..71121 - 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LO739_RS23365 (LO739_23360) 71695..72045 + 351 WP_000493378.1 hypothetical protein -
LO739_RS23370 (LO739_23365) 72096..72839 + 744 WP_000129823.1 hypothetical protein -
LO739_RS23375 (LO739_23370) 72836..73612 + 777 WP_000015958.1 site-specific integrase -
LO739_RS23380 (LO739_23375) 73670..73927 - 258 WP_000764642.1 hypothetical protein -
LO739_RS23385 (LO739_23380) 74056..74160 - 105 WP_032409716.1 hypothetical protein -
LO739_RS23390 (LO739_23385) 74690..75556 + 867 WP_004118283.1 replication initiation protein -
LO739_RS23395 (LO739_23390) 75733..76002 - 270 WP_000339857.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / qnrS1 / dfrA14 / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / mph(A) / mph(E) / msr(E) / floR / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib - 1..76208 76208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T225209 WP_000754566.1 NZ_CP087281:c70894-70478 [Leclercia adecarboxylata]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T225209 NZ_CP121073:c4183409-4183125 [Salmonella enterica subsp. enterica serovar Infantis]
ATGAATTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT225209 WP_001261276.1 NZ_CP087281:c71121-70891 [Leclercia adecarboxylata]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT225209 NZ_CP121073:c4183641-4183399 [Salmonella enterica subsp. enterica serovar Infantis]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCCTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGAATTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References