Detailed information of TA system TA225199

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 2346214..2346788
• Replicon: chromosome
• Accession: NZ_CP087280
• Organism: Leclercia adecarboxylata strain SH19PE29

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LO739_RS11110
• Protein ID: WP_059307894.1
• Coordinates: 2346462..2346788 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A6G7TED5
• Locus tag: LO739_RS11105
• Protein ID: WP_059307895.1
• Coordinates: 2346214..2346462 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO739_RS11090 (LO739_11090)	2343118..2344662	+	1545	WP_059307898.1	nitrate reductase subunit beta	-
LO739_RS11095 (LO739_11095)	2344662..2345357	+	696	WP_032618186.1	nitrate reductase molybdenum cofactor assembly chaperone	-
LO739_RS11100 (LO739_11100)	2345354..2346034	+	681	WP_059307896.1	respiratory nitrate reductase subunit gamma	-
LO739_RS11105 (LO739_11105)	2346214..2346462	+	249	WP_059307895.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LO739_RS11110 (LO739_11110)	2346462..2346788	+	327	WP_059307894.1	endoribonuclease MazF	Toxin
LO739_RS11115 (LO739_11115)	2346817..2347662	-	846	WP_059307893.1	N-hydroxyarylamine O-acetyltransferase	-
LO739_RS11120 (LO739_11120)	2347859..2349514	-	1656	WP_230431501.1	iron ABC transporter permease	-
LO739_RS11125 (LO739_11125)	2349553..2350521	-	969	WP_230431502.1	ABC transporter substrate-binding protein	-
LO739_RS11130 (LO739_11130)	2350518..2351210	-	693	WP_191152655.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-107)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11875.64 Da
• Isoelectric Point: 7.8831

>T225199 WP_059307894.1 NZ_CP087280:2346462-2346788 [Leclercia adecarboxylata]
MDYIPEAGDIVWLDFDPQSGHEQAGHRPALVISPATYNGRTGLMLCCPVTSQIKNYPFEVVISGARRNVALADQIKSLDW
RARHAVKKGTATAEELDAVRMRAKVLIG

Nucleotide

• Length: 327 bp

>T225199 NZ_CP121073:204572-205057 [Salmonella enterica subsp. enterica serovar Infantis]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9137.39 Da
• Isoelectric Point: 4.4777

>AT225199 WP_059307895.1 NZ_CP087280:2346214-2346462 [Leclercia adecarboxylata]
MPQVTVKKWGNSPSVRLPVAVMREAELHVDDVVNVNVDEEGRIILTPVRKDEPTLESLLAAITDDNLHHEVTFGEPQGKE
LL

Nucleotide

• Length: 249 bp

>AT225199 NZ_CP121073:204297-204584 [Salmonella enterica subsp. enterica serovar Infantis]
ATGCTATACAAAGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCAGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A6G7TED5