Detailed information of TA system TA225195

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 726501..727144
• Replicon: chromosome
• Accession: NZ_CP087280
• Organism: Leclercia adecarboxylata strain SH19PE29

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: LO739_RS03380
• Protein ID: WP_015387203.1
• Coordinates: 726501..726917 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A3E1ZVJ5
• Locus tag: LO739_RS03385
• Protein ID: WP_032616516.1
• Coordinates: 726914..727144 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO739_RS03360 (LO739_03360)	722548..723138	-	591	WP_059308736.1	TetR/AcrR family transcriptional regulator	-
LO739_RS03365 (LO739_03365)	723263..724138	-	876	WP_059308735.1	NmrA family NAD(P)-binding protein	-
LO739_RS03370 (LO739_03370)	724382..725287	-	906	WP_059308734.1	SDR family oxidoreductase	-
LO739_RS03375 (LO739_03375)	725406..726302	+	897	WP_059308733.1	AraC family transcriptional regulator	-
LO739_RS03380 (LO739_03380)	726501..726917	-	417	WP_015387203.1	type II toxin-antitoxin system VapC family toxin	Toxin
LO739_RS03385 (LO739_03385)	726914..727144	-	231	WP_032616516.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LO739_RS03390 (LO739_03390)	727548..727700	+	153	WP_059308732.1	Hok/Gef family protein	-
LO739_RS03395 (LO739_03395)	727792..728397	-	606	WP_059308731.1	3-isopropylmalate dehydratase small subunit	-
LO739_RS03400 (LO739_03400)	728408..729808	-	1401	WP_032616519.1	3-isopropylmalate dehydratase large subunit	-
LO739_RS03405 (LO739_03405)	729811..730902	-	1092	WP_059308730.1	3-isopropylmalate dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14924.31 Da
• Isoelectric Point: 7.2449

>T225195 WP_015387203.1 NZ_CP087280:c726917-726501 [Leclercia adecarboxylata]
VIKTYMLDTNICSFIMREQPEAVIKRLEQAVLRNHRIVVSAITYAEMRFGAIGKKASPRHGLLVEAFCARLDAILAWDRA
AVDATTEIKAALTAAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWAN

Nucleotide

• Length: 417 bp

>T225195 NZ_CP121071:14789-15121 [Salmonella enterica subsp. enterica serovar Infantis]
ATGGACAGAGGGGAAATCTGGCTCGTCTCACTGGATCCTACAGCCGGACATGAACAAAGCGGAAAACGTCCGGTACTTAT
CGTTTCTCCGGCATCATTTAATAAGTTCACCCGGTTACCCGTTGTCGTTCCCGTCACCAGCGGCGGAGACTTTGCCCGCA
CAGCCGGTTTTACCGTCTCCCTGGACGGCGCGGGAACAAAAACAACGGGTGTTATTCGCTGCGACCAGCCCAGAACGATT
GATATGGGTGCCCGGAATGGTAAGCGTCTGGAACGTATACCCGAAGCTGTCGTAAATGAAGTGCTGGCCCGACTGGAAGC
CATTCTGAGCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8687.72 Da
• Isoelectric Point: 4.4563

>AT225195 WP_032616516.1 NZ_CP087280:c727144-726914 [Leclercia adecarboxylata]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFTQLEKADSDFMSERGDVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT225195 NZ_CP121071:14531-14788 [Salmonella enterica subsp. enterica serovar Infantis]
ATGTATACCACTCGTCTGAAAAAGGTTGGCGGATCCGTCATGCTGGCGGTTCCTCCCGCCGTGTTGAAAACGCTGGCTCT
GTCGACAGACAGCGAAGTGGGAATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAACTGCTGGCACAGTGCGATCCGCACGCCGAAATGAGCGATGAGGATAGGGAATGGATGGATGCGCCTGCAGTG
GGTAAGGAGATCCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3E1ZVJ5