Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-MazE
Location 3009702..3010335 Replicon chromosome
Accession NZ_CP087277
Organism Ralstonia solanacearum strain FJ1003

Toxin (Protein)


Gene name vapC Uniprot ID M4UIT8
Locus tag LOH50_RS14355 Protein ID WP_016725172.1
Coordinates 3009702..3010115 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q8XUX7
Locus tag LOH50_RS14360 Protein ID WP_011002949.1
Coordinates 3010102..3010335 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOH50_RS14335 3005084..3006820 + 1737 WP_058908046.1 ABC transporter substrate-binding protein -
LOH50_RS14340 3006982..3008478 + 1497 WP_011002945.1 glycerol kinase GlpK -
LOH50_RS14345 3008539..3008910 - 372 WP_011002946.1 hypothetical protein -
LOH50_RS14350 3009101..3009727 + 627 WP_058908047.1 DUF1415 domain-containing protein -
LOH50_RS14355 3009702..3010115 - 414 WP_016725172.1 PIN domain-containing protein Toxin
LOH50_RS14360 3010102..3010335 - 234 WP_011002949.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
LOH50_RS14365 3010392..3011999 - 1608 WP_058908048.1 glucan biosynthesis protein -
LOH50_RS14370 3012212..3013444 + 1233 WP_058908049.1 AGE family epimerase/isomerase -
LOH50_RS14375 3013452..3014747 + 1296 WP_011002952.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-116)

Antitoxin

SpoVT-AbrB domain

(5-37)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 15087.42 Da        Isoelectric Point: 5.5348

>T225193 WP_016725172.1 NZ_CP087277:c3010115-3009702 [Ralstonia solanacearum]
MPATEAFFDSNVVLYLLSADTAKADAAETLLMTGGVVSVQVLNETTHVMRRKLAMPWHAIETVQEAVRAQCRVEPLTLET
HDLGRRIAERYGLSVYDALIVAAALLAGCNVLYSEDMQHGLVIEQHLRIVNPFPARA

Download         Length: 414 bp

>T225193 NZ_CP121070:c4660374-4660063 [Salmonella enterica subsp. enterica serovar Infantis]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8790.98 Da        Isoelectric Point: 8.7287

>AT225193 WP_011002949.1 NZ_CP087277:c3010335-3010102 [Ralstonia solanacearum]
MQVSKWGNSLAIRLPAAVVEALQLHEGDDVEVVIAGERSFGVRRKRAARELFDRIHQYRGKLPADFIFERDQANARD

Download         Length: 234 bp

>AT225193 NZ_CP121070:c4660062-4659772 [Salmonella enterica subsp. enterica serovar Infantis]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M4UIT8

Antitoxin

Source ID Structure
AlphaFold DB A0A223GR76

References