Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 109688..110424 Replicon plasmid plas1
Accession NZ_CP087273
Organism Klebsiella pneumoniae strain ATCC BAA 2146

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag LN739_RS27510 Protein ID WP_003026803.1
Coordinates 109688..110170 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag LN739_RS27515 Protein ID WP_003026799.1
Coordinates 110158..110424 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN739_RS27490 (LN739_27490) 105042..106805 - 1764 WP_004200842.1 DUF262 domain-containing protein -
LN739_RS27495 (LN739_27495) 107006..107197 - 192 Protein_103 helix-turn-helix domain-containing protein -
LN739_RS27500 (LN739_27500) 107423..108055 + 633 WP_001567369.1 hypothetical protein -
LN739_RS27505 (LN739_27505) 108084..109487 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
LN739_RS27510 (LN739_27510) 109688..110170 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
LN739_RS27515 (LN739_27515) 110158..110424 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
LN739_RS27520 (LN739_27520) 110669..111103 - 435 WP_001567367.1 cell envelope integrity protein TolA -
LN739_RS27525 (LN739_27525) 111150..111698 - 549 WP_001567366.1 thioredoxin fold domain-containing protein -
LN739_RS27530 (LN739_27530) 112150..112500 + 351 WP_004200891.1 hypothetical protein -
LN739_RS27535 (LN739_27535) 113399..114937 - 1539 WP_004201219.1 IS66-like element ISKpn24 family transposase -
LN739_RS27540 (LN739_27540) 114986..115333 - 348 WP_000612626.1 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / tet(A) - 1..117755 117755
- inside IScluster/Tn - - 108084..115734 7650


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T225188 WP_003026803.1 NZ_CP087273:c110170-109688 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T225188 NZ_CP121070:c2299249-2298965 [Salmonella enterica subsp. enterica serovar Infantis]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT225188 WP_003026799.1 NZ_CP087273:c110424-110158 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT225188 NZ_CP121070:c2299487-2299239 [Salmonella enterica subsp. enterica serovar Infantis]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGAGGGCGCTCCAGTTGCCATTCTTAACCGTAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References