Detailed information of TA system TA225169

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 2937..3490
• Replicon: plasmid pHD6448-5
• Accession: NZ_CP087271
• Organism: Aeromonas veronii strain HD6448

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: KXU59_RS24660
• Protein ID: WP_094461470.1
• Coordinates: 3182..3490 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: KXU59_RS24655
• Protein ID: WP_181411537.1
• Coordinates: 2937..3182 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KXU59_RS24635 (LOS88_24635)	371..604	+	234	WP_113740815.1	MbeD/MobD family mobilization/exclusion protein	-
KXU59_RS24640 (LOS88_24640)	601..816	+	216	WP_197971075.1	hypothetical protein	-
KXU59_RS24645 (LOS88_24645)	1150..2097	+	948	WP_031285326.1	IS30 family transposase	-
KXU59_RS24650 (LOS88_24650)	2376..2594	-	219	WP_181411541.1	hypothetical protein	-
KXU59_RS24655 (LOS88_24655)	2937..3182	+	246	WP_181411537.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
KXU59_RS24660 (LOS88_24660)	3182..3490	+	309	WP_094461470.1	CcdB family protein	Toxin
KXU59_RS24665 (LOS88_24665)	3494..3709	+	216	WP_094461471.1	hypothetical protein	-
KXU59_RS24670 (LOS88_24670)	3924..4901	+	978	WP_181562255.1	replication initiation protein	-
KXU59_RS24675 (LOS88_24675)	5052..5342	-	291	WP_104456155.1	hypothetical protein	-
KXU59_RS24680 (LOS88_24680)	5541..5879	+	339	WP_147095875.1	MobC family plasmid mobilization relaxosome protein	-
KXU59_RS24685 (LOS88_24685)	5860..6654	+	795	WP_245147302.1	relaxase/mobilization nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..6668	6668
	-	flank	IS/Tn	-	-	1225..2097	872

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (11-81)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11524.32 Da
• Isoelectric Point: 5.0774

>T225169 WP_094461470.1 NZ_CP087271:3182-3490 [Aeromonas veronii]
MQFTIYRNKGNARDYPYLLNVQSDIIDELNTRMVIPLVPLAAFIGRPAQRLNPVISVEGKKHLVLTQEMASVRLGQLGEE
VTDVQESRQLIKDSIDFLLDGV

Nucleotide

• Length: 309 bp

>T225169 NZ_CP121068:c1570921-1570505 [Salmonella enterica subsp. enterica serovar Infantis]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9198.43 Da
• Isoelectric Point: 10.6626

>AT225169 WP_181411537.1 NZ_CP087271:2937-3182 [Aeromonas veronii]
MVTATMRIRSKRTVSVTLEPSLLERARAAGINLSATLATALQHEISGSEAERWKRDNREAIQTLNRMVEERGLFSDDHRT
F

Nucleotide

• Length: 246 bp

>AT225169 NZ_CP121068:c1571148-1570918 [Salmonella enterica subsp. enterica serovar Infantis]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA