Detailed information of TA system TA225161

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 48903..49480
• Replicon: plasmid unnamed
• Accession: NZ_CP087265
• Organism: Listeria monocytogenes strain s2020TJ

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: H1G8D6
• Locus tag: LOS95_RS15015
• Protein ID: WP_003728465.1
• Coordinates: 49139..49480 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H1G8D5
• Locus tag: LOS95_RS15010
• Protein ID: WP_003728464.1
• Coordinates: 48903..49145 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOS95_RS14965 (LOS95_14965)	44175..44891	-	717	WP_003731678.1	AP2 domain-containing protein	-
LOS95_RS14970 (LOS95_14970)	44907..45629	-	723	WP_003728517.1	CRISPR-associated protein Cas5	-
LOS95_RS14975 (LOS95_14975)	45633..46058	-	426	WP_003728518.1	hypothetical protein	-
LOS95_RS14980 (LOS95_14980)	46059..46286	-	228	WP_003769261.1	hypothetical protein	-
LOS95_RS14985 (LOS95_14985)	46429..46677	-	249	WP_023553717.1	hypothetical protein	-
LOS95_RS14990 (LOS95_14990)	47076..47453	-	378	WP_003769263.1	DUF3850 domain-containing protein	-
LOS95_RS14995 (LOS95_14995)	47472..48134	-	663	WP_003731676.1	DUF2786 domain-containing protein	-
LOS95_RS15000 (LOS95_15000)	48134..48460	-	327	WP_003728462.1	hypothetical protein	-
LOS95_RS15005 (LOS95_15005)	48606..48842	+	237	WP_003769265.1	hypothetical protein	-
LOS95_RS15010 (LOS95_15010)	48903..49145	+	243	WP_003728464.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LOS95_RS15015 (LOS95_15015)	49139..49480	+	342	WP_003728465.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LOS95_RS15020 (LOS95_15020)	49673..51808	-	2136	WP_003728466.1	heavy metal translocating P-type ATPase	-
LOS95_RS15025 (LOS95_15025)	51808..52167	-	360	WP_003728467.1	Cd(II)-sensing metalloregulatory transcriptional repressor CadC	-
LOS95_RS15030 (LOS95_15030)	52447..53001	+	555	WP_003728468.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	ClpL	-	1..57623	57623

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-105)

Antitoxin

• SpoVT-AbrB domain (7-46)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12827.74 Da
• Isoelectric Point: 9.2830

>T225161 WP_003728465.1 NZ_CP087265:49139-49480 [Listeria monocytogenes]
MVKQGDIIKINLNPKQGHEQQGYRPYICLSHHLVSDYANIAIFAPISNTPRTYPLYVPLTGTTTTGKVLLDQLVTIDYNA
RKHQYVETVSDPLLTKLLDTVKVIFQKNERSTN

Nucleotide

• Length: 342 bp

>T225161 NZ_CP121067:14789-15121 [Salmonella enterica subsp. enterica serovar Infantis]
ATGGACAGAGGGGAAATCTGGCTCGTCTCACTGGATCCTACAGCCGGACATGAACAAAGCGGAAAACGTCCGGTACTTAT
CGTTTCTCCGGCATCATTTAATAAGTTCACCCGGTTACCCGTTGTCGTTCCCGTCACCAGCGGCGGAGACTTTGCCCGCA
CAGCCGGTTTTACCGTCTCCCTGGACGGCGCGGGAACAAAAACAACGGGTGTTATTCGCTGCGACCAGCCCAGAACGATT
GATATGGGTGCCCGGAATGGTAAGCGTCTGGAACGTATACCCGAAGCTGTCGTAAATGAAGTGCTGGCCCGACTGGAAGC
CATTCTGAGCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9207.48 Da
• Isoelectric Point: 4.3867

>AT225161 WP_003728464.1 NZ_CP087265:48903-49145 [Listeria monocytogenes]
MTTVSVKKWGNSKAVRLPNNLLNALDIKENDDLKVEVINNQMILTKTAIDLRIEDLFANYQGEPFQTELQTFEPIGDEKW

Nucleotide

• Length: 243 bp

>AT225161 NZ_CP121067:14531-14788 [Salmonella enterica subsp. enterica serovar Infantis]
ATGTATACCACTCGTCTGAAAAAGGTTGGCGGATCCGTCATGCTGGCGGTTCCTCCCGCCGTGTTGAAAACGCTGGCTCT
GTCGACAGACAGCGAAGTGGGAATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAACTGCTGGCACAGTGCGATCCGCACGCCGAAATGAGCGATGAGGATAGGGAATGGATGGATGCGCCTGCAGTG
GGTAAGGAGATCCTGTAA

Structures

Toxin

• AlphaFold DB: H1G8D6

Antitoxin

• AlphaFold DB: H1G8D5