Detailed information of TA system TA225148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1230714..1231339
• Replicon: chromosome
• Accession: NZ_CP087226
• Organism: Dickeya fangzhongdai strain B16

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: E0SD87
• Locus tag: QR68_RS05520
• Protein ID: WP_009114000.1
• Coordinates: 1230714..1230917 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: QR68_RS05525
• Protein ID: WP_038918199.1
• Coordinates: 1230971..1231339 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QR68_RS05490 (QR68_05490)	1226352..1226690	+	339	WP_002208627.1	P-II family nitrogen regulator	-
QR68_RS05495 (QR68_05495)	1226733..1228025	+	1293	WP_038661206.1	ammonium transporter AmtB	-
QR68_RS05500 (QR68_05500)	1228118..1228981	-	864	WP_038918195.1	acyl-CoA thioesterase II	-
QR68_RS05505 (QR68_05505)	1229234..1229794	+	561	WP_038918196.1	YbaY family lipoprotein	-
QR68_RS05510 (QR68_05510)	1229823..1230152	-	330	WP_038918197.1	MGMT family protein	-
QR68_RS05520 (QR68_05520)	1230714..1230917	-	204	WP_009114000.1	HHA domain-containing protein	Toxin
QR68_RS05525 (QR68_05525)	1230971..1231339	-	369	WP_038918199.1	Hha toxicity modulator TomB	Antitoxin
QR68_RS05530 (QR68_05530)	1231847..1231990	-	144	WP_013316871.1	type B 50S ribosomal protein L36	-
QR68_RS05535 (QR68_05535)	1232006..1232257	-	252	WP_038661187.1	type B 50S ribosomal protein L31	-
QR68_RS05540 (QR68_05540)	1232428..1235574	-	3147	WP_038918200.1	efflux RND transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8133.51 Da
• Isoelectric Point: 8.8500

>T225148 WP_009114000.1 NZ_CP087226:c1230917-1230714 [Dickeya fangzhongdai]
MKKIDYLMRLRKCTTIDTLERVIEKNKYELSNDELEMFYSAADHRLAELTMNKLYDKVPTAVWKYVR

Nucleotide

• Length: 204 bp

>T225148 NZ_CP121005:c4002-3721 [Salmonella enterica]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14181.02 Da
• Isoelectric Point: 5.1663

>AT225148 WP_038918199.1 NZ_CP087226:c1231339-1230971 [Dickeya fangzhongdai]
MDEYTPQHYDIAQLRFLCENLHDESIATLGDSSHGWVNDPTSAVNLQLNELIEHIAAFVVTYKIKYPHEAALCERVEKYL
DDTYILFSNYGINDSELQKWKKSKSQLFRMFSEKSICTVVKT

Nucleotide

• Length: 369 bp

>AT225148 NZ_CP121005:c4243-3992 [Salmonella enterica]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: A0A1X3RNQ0