Detailed information of TA system TA225145

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 575594..576375
• Replicon: chromosome
• Accession: NZ_CP087226
• Organism: Dickeya fangzhongdai strain B16

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: QR68_RS02660
• Protein ID: WP_038917749.1
• Coordinates: 575884..576375 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A1Y0L5F0
• Locus tag: QR68_RS02655
• Protein ID: WP_038917748.1
• Coordinates: 575594..575887 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QR68_RS02635 (QR68_02635)	571604..572716	+	1113	WP_038917743.1	glycosyltransferase family 4 protein	-
QR68_RS02640 (QR68_02640)	572735..573142	+	408	WP_038917744.1	hypothetical protein	-
QR68_RS02645 (QR68_02645)	573181..574575	+	1395	WP_038917746.1	DUF4832 domain-containing protein	-
QR68_RS02650 (QR68_02650)	574602..575102	+	501	WP_038917747.1	serine acetyltransferase	-
QR68_RS02655 (QR68_02655)	575594..575887	+	294	WP_038917748.1	DUF1778 domain-containing protein	Antitoxin
QR68_RS02660 (QR68_02660)	575884..576375	+	492	WP_038917749.1	GNAT family N-acetyltransferase	Toxin
QR68_RS02665 (QR68_02665)	576534..576776	+	243	WP_071605145.1	hypothetical protein	-
QR68_RS02670 (QR68_02670)	576989..581071	+	4083	WP_038917751.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	575338..589556	14218

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (75-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17628.53 Da
• Isoelectric Point: 8.2830

>T225145 WP_038917749.1 NZ_CP087226:575884-576375 [Dickeya fangzhongdai]
MISAPEPLRAEHVLSSFCCGVESMDNWLKQRAMKNQVTGASRTFVSCNDSKVLAYYSLASSVVATNAAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEAREFYLRVGFEPSPIDPMMLMVTLGDLVG
SVI

Nucleotide

• Length: 492 bp

>T225145 NZ_CP121003:c4488952-4488641 [Salmonella enterica]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGAGGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10872.44 Da
• Isoelectric Point: 10.1968

>AT225145 WP_038917748.1 NZ_CP087226:575594-575887 [Dickeya fangzhongdai]
MPATNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLARLDQAPSPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT225145 NZ_CP121003:c4488640-4488350 [Salmonella enterica]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A1Y0L5F0