Detailed information of TA system TA225144

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 425386..426422
• Replicon: chromosome
• Accession: NZ_CP087226
• Organism: Dickeya fangzhongdai strain B16

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: QR68_RS01945
• Protein ID: WP_038917617.1
• Coordinates: 425847..426422 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: QR68_RS01940
• Protein ID: WP_038917616.1
• Coordinates: 425386..425847 (+)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QR68_RS01920 (QR68_01920)	420556..421479	+	924	WP_038917612.1	type I-E CRISPR-associated endonuclease Cas1e	-
QR68_RS01925 (QR68_01925)	421476..421769	+	294	WP_038917614.1	type I-E CRISPR-associated endoribonuclease Cas2e	-
QR68_RS01930 (QR68_01930)	423162..424838	-	1677	WP_038917615.1	methyl-accepting chemotaxis protein	-
QR68_RS01935 (QR68_01935)	425010..425120	-	111	Protein_372	hypothetical protein	-
QR68_RS01940 (QR68_01940)	425386..425847	+	462	WP_038917616.1	helix-turn-helix domain-containing protein	Antitoxin
QR68_RS01945 (QR68_01945)	425847..426422	+	576	WP_038917617.1	PIN domain-containing protein	Toxin
QR68_RS01950 (QR68_01950)	426646..427098	+	453	WP_038917619.1	YhbP family protein	-
QR68_RS01955 (QR68_01955)	427210..427674	-	465	WP_038917620.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
QR68_RS01960 (QR68_01960)	427773..429911	-	2139	WP_038917621.1	anaerobic ribonucleoside-triphosphate reductase	-
QR68_RS01965 (QR68_01965)	430291..431199	+	909	WP_038917622.1	homocysteine S-methyltransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-115)

Antitoxin

• SinI-like, DNA-binding domain (84-131)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.93 Da
• Isoelectric Point: 4.8531

>T225144 WP_038917617.1 NZ_CP087226:425847-426422 [Dickeya fangzhongdai]
MTHTPYPVVLDACVIYPSLLRDLLIHLGLTGLYQPKWTAIIEDEWQRNLLLNRPDLTPEQISRTRELMNQAIPDAMITGF
EALIPGITLPDPDDQHVVAAAIRSNAEIIVTFNLKDFPHPEIDNFGIEALHPDDFITDLLDLNQALVLSAVSRQRHSMRK
PPKSVDEYLDALLRQSLPQTVKELSKFHALI

Nucleotide

• Length: 576 bp

>T225144 NZ_CP121003:c4179715-4179224 [Salmonella enterica]
ATGATCTCCACCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATAGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGCGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTCGCTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGTGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTATCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 16913.27 Da
• Isoelectric Point: 6.2221

>AT225144 WP_038917616.1 NZ_CP087226:425386-425847 [Dickeya fangzhongdai]
MTNSILDSLSLPAPGEIEAAVRGQRELAAYLSTKMETQKISIQDADNNTRQVELPTSALTLLMSILGELAVGNAVQVVPV
HAELTTQEAANILNVSRPHMVKLLEDGKLPFHKTGRHRRVLFADLMDYKHQRDNESSRAMQALADHSQELGLY

Nucleotide

• Length: 462 bp

>AT225144 NZ_CP121003:c4180005-4179712 [Salmonella enterica]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATTGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACTGACTTTGTGCTGGAAGCTGCCCGTGCTGCCGCCGAGGAAGCGCTGA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGAGTTTCTCGTTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA