Detailed information of TA system TA022514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazE-yefM/MazF-Phd
• Location: 3149531..3150243
• Replicon: chromosome
• Accession: NC_009943
• Organism: Desulfococcus oleovorans Hxd3

Toxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: DOLE_RS13380
• Protein ID: WP_012176020.1
• Coordinates: 3149531..3149860 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A8ZWS8
• Locus tag: DOLE_RS13385
• Protein ID: WP_012176021.1
• Coordinates: 3150001..3150243 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DOLE_RS13350	3144854..3145897	+	1044	WP_153304433.1	hypothetical protein	-
DOLE_RS13355	3146491..3147264	+	774	WP_012176018.1	DUF4393 domain-containing protein	-
DOLE_RS13360	3147398..3147925	+	528	WP_153304434.1	hypothetical protein	-
DOLE_RS13365	3148007..3148312	+	306	WP_041280573.1	hypothetical protein	-
DOLE_RS13375	3149283..3149537	+	255	WP_041280574.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
DOLE_RS13380	3149531..3149860	+	330	WP_012176020.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DOLE_RS13385	3150001..3150243	+	243	WP_012176021.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
DOLE_RS13390	3150243..3150503	+	261	WP_012176022.1	Txe/YoeB family addiction module toxin	-
DOLE_RS13395	3150666..3151655	+	990	WP_012176023.1	NAD-dependent epimerase/dehydratase family protein	-
DOLE_RS13400	3151631..3152140	+	510	WP_208596980.1	SRPBCC family protein	-
DOLE_RS13405	3152159..3153100	+	942	WP_208596981.1	hypothetical protein	-
DOLE_RS13410	3153181..3153525	+	345	WP_012176026.1	hypothetical protein	-
DOLE_RS13415	3153670..3153861	+	192	WP_012176027.1	ferredoxin	-
DOLE_RS13420	3153945..3154520	+	576	WP_012176028.1	rubrerythrin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-107)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-69)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12344.57 Da
• Isoelectric Point: 10.2226

>T22514 WP_012176020.1 NC_009943:3149531-3149860 [Desulfococcus oleovorans Hxd3]
MVKKEVQRFEIWLVQLDPTQGSEIKKTRPCVVVSPDEMAALKTAIVAPMTSKGFSYPTRVQYTFQGKKGLILLDQLRAVD
KSRLIQKLGVISQNSQIKITNCLQELFAY

Nucleotide

• Length: 330 bp

>T22514 NC_009943:3149531-3149860 [Desulfococcus oleovorans Hxd3]
ATGGTAAAAAAAGAGGTTCAAAGATTTGAGATTTGGCTGGTTCAACTTGATCCGACACAAGGTTCTGAAATTAAAAAAAC
AAGGCCTTGTGTTGTTGTCTCCCCTGATGAAATGGCTGCTCTGAAGACCGCGATTGTTGCCCCGATGACATCCAAAGGAT
TTAGCTATCCAACCAGAGTCCAATATACATTTCAGGGTAAAAAAGGATTGATCTTGCTTGACCAACTGAGAGCCGTTGAC
AAATCCAGGTTAATTCAAAAACTTGGTGTAATTTCCCAAAATTCGCAGATTAAAATTACCAATTGTTTGCAAGAGCTTTT
CGCGTATTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8973.16 Da
• Isoelectric Point: 4.2340

>AT22514 WP_012176021.1 NC_009943:3150001-3150243 [Desulfococcus oleovorans Hxd3]
MPTLTATEARSKLYGLIDETAASHEPIIIKGKRGNAVLISEDDWRSIQETIYLLNIPGMRESIRDGLATPVEECTEELDW

Nucleotide

• Length: 243 bp

>AT22514 NC_009943:3150001-3150243 [Desulfococcus oleovorans Hxd3]
ATGCCGACATTAACTGCCACAGAAGCAAGGTCAAAACTCTATGGCCTGATCGATGAAACAGCTGCGTCCCATGAGCCAAT
AATTATTAAAGGCAAGCGCGGTAATGCAGTGCTTATCTCTGAAGATGACTGGCGCTCTATCCAGGAAACAATTTATCTCC
TGAACATTCCAGGTATGCGGGAATCAATACGAGACGGTTTAGCCACCCCCGTCGAGGAGTGTACAGAGGAACTTGATTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A8ZWS8