Detailed information of TA system TA225135

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1629491..1630530
• Replicon: chromosome
• Accession: NZ_CP087204
• Organism: Pseudomonas sp. B21-054

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: LOY70_RS07235
• Protein ID: WP_123414765.1
• Coordinates: 1629491..1630066 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: LOY70_RS07240
• Protein ID: WP_265035033.1
• Coordinates: 1630063..1630530 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY70_RS07210 (LOY70_07190)	1626235..1626627	+	393	WP_265035026.1	TauD/TfdA family dioxygenase	-
LOY70_RS07215 (LOY70_07195)	1626624..1626974	+	351	WP_265035028.1	hypothetical protein	-
LOY70_RS07220 (LOY70_07200)	1627026..1628188	+	1163	WP_265035029.1	IS3 family transposase	-
LOY70_RS07225 (LOY70_07205)	1628213..1628815	+	603	WP_265035031.1	hypothetical protein	-
LOY70_RS07230 (LOY70_07210)	1629209..1629385	-	177	Protein_1428	transposase domain-containing protein	-
LOY70_RS07235 (LOY70_07215)	1629491..1630066	-	576	WP_123414765.1	PIN domain-containing protein	Toxin
LOY70_RS07240 (LOY70_07220)	1630063..1630530	-	468	WP_265035033.1	helix-turn-helix domain-containing protein	Antitoxin
LOY70_RS07250 (LOY70_07230)	1631366..1632250	-	885	WP_265035035.1	alpha/beta hydrolase	-
LOY70_RS07255 (LOY70_07235)	1632562..1633254	-	693	WP_265035037.1	pseudouridine synthase	-
LOY70_RS07260 (LOY70_07240)	1633290..1633508	-	219	WP_123414761.1	cysteine-rich CWC family protein	-
LOY70_RS07265 (LOY70_07245)	1633501..1634112	-	612	Protein_1434	GGDEF domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21759.86 Da
• Isoelectric Point: 5.5793

>T225135 WP_123414765.1 NZ_CP087204:c1630066-1629491 [Pseudomonas sp. B21-054]
MRHSPYTAVYDANVLYPAPLRDFLMRLALTGAYRARWSAQIHDEWKRNLLVKRPDLTVDQVNRTSALMDRAIPDALVTDY
ESIVAQLDLPDADDRHVLAAAIKCNASAIVTFNLKDFPQAALDSWGIEALHPDEFIVDLWDLDQAAVVEAAQRQRASLKN
PPHTVREYLDKLLQQKLPETVKLLSHFEYVL

Nucleotide

• Length: 576 bp

>T225135 NZ_CP121003:836166-836564 [Salmonella enterica]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17377.85 Da
• Isoelectric Point: 5.0703

>AT225135 WP_265035033.1 NZ_CP087204:c1630530-1630063 [Pseudomonas sp. B21-054]
MTTADLTRAVLPDEREIAVAVESSRQIAAFLSAKQETQRIELVDGEQQRQVVELPIYAIRLLGEILSELALGNTVKVVPI
HAELTTQEGADLLNVSRPHLVKLLDEGVMPHTKTGRHRRIKFADLMEYKNRREQDSHAILDELAAQAQELGMGYE

Nucleotide

• Length: 468 bp

>AT225135 NZ_CP121003:835939-836166 [Salmonella enterica]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA