Detailed information of TA system TA225127

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3843545..3844584
• Replicon: chromosome
• Accession: NZ_CP087202
• Organism: Pseudomonas asplenii strain B21-058

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: LOY63_RS16460
• Protein ID: WP_010453102.1
• Coordinates: 3844009..3844584 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A1H6P4A9
• Locus tag: LOY63_RS16455
• Protein ID: WP_010453103.1
• Coordinates: 3843545..3844012 (+)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY63_RS16435 (LOY63_16425)	3839001..3839903	-	903	WP_265084190.1	LysR family transcriptional regulator	-
LOY63_RS16440 (LOY63_16430)	3840049..3841251	+	1203	WP_019361934.1	MFS transporter	-
LOY63_RS16445 (LOY63_16435)	3841345..3842340	+	996	WP_010453108.1	MBL fold metallo-hydrolase	-
LOY63_RS16450 (LOY63_16440)	3842366..3843220	+	855	WP_010453106.1	alpha/beta hydrolase	-
LOY63_RS16455 (LOY63_16445)	3843545..3844012	+	468	WP_010453103.1	helix-turn-helix domain-containing protein	Antitoxin
LOY63_RS16460 (LOY63_16450)	3844009..3844584	+	576	WP_010453102.1	PIN domain-containing protein	Toxin
LOY63_RS16465 (LOY63_16455)	3844779..3845966	+	1188	WP_265084191.1	MFS transporter	-
LOY63_RS16470 (LOY63_16460)	3845994..3846911	-	918	WP_010453098.1	LysR substrate-binding domain-containing protein	-
LOY63_RS16475 (LOY63_16465)	3847095..3847844	+	750	WP_010453095.1	SDR family NAD(P)-dependent oxidoreductase	-
LOY63_RS16480 (LOY63_16470)	3847931..3849229	+	1299	WP_265084192.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3843545..3882503	38958

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-115)

Antitoxin

• SinI-like, DNA-binding domain (84-130)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21498.58 Da
• Isoelectric Point: 4.6018

>T225127 WP_010453102.1 NZ_CP087202:3844009-3844584 [Pseudomonas asplenii]
MRHSPFTAVYDANVLYPAPLRDLLMHLALTGIYRARWTVQIHEEWKRNLLINRPDLTQTQLDRTSMAMDRAIPDALVTGY
ASLYSGLTLPDPDDRHILAAAIKCSASVIVTFNLKDFPPDTLQPFDIEAVHPDDFIADLFDLDRAAVLEAVQAQRASLKN
PPYSARELLEQLLAQGLAQSVELLSEYEQLI

Nucleotide

• Length: 576 bp

>T225127 NZ_CP120979:c91932-91582 [Moellerella wisconsensis]
ATGTGGGAAATATTAACGAGAGATCTCTTTGATTCTTGGTTCGAAGAGCAAAATGAGGAAACTCAGATTGAAGTGTTGGC
AGTGTTAATGATTTTACGTGAGGATGGCCCCAATCTTGGACGCCCTCAAGTTGACACCCTGAAGGGCTCACAGTTTCCCA
ACATGAAGGAACTGCGAATTCAGGTTGGTGGTCATCCGATTAGAGCGTGTTTCGCATTTGACCCAATTCGTCGTGGCATT
GTGTTATGTGCCGGTGATAAGAAAGGAAAAGATGAAACCCGTTTCTACAAGAAATTAATCAAGATGGCCGATGCGGAATA
TGCAGCGCACTTATTAGAGCAGGAGAAATAA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17562.08 Da
• Isoelectric Point: 5.9290

>AT225127 WP_010453103.1 NZ_CP087202:3843545-3844012 [Pseudomonas asplenii]
MTTTDLNATMLPDEREIAAAVESRRQLAAFLSTKLDTQRIEILDDQHQPHTIQLPVSALRLLDEILSELSMGNAVKVVPI
HAELTTQEGADLLNVSRPHLVKLLDENVIPHTKVGRHRRVKFADLMDYKQRRTAESRQAMDELATQAQELKMGYE

Nucleotide

• Length: 468 bp

>AT225127 NZ_CP120979:c91580-91281 [Moellerella wisconsensis]
ATGTCCACTTTAAATGAACTACTTGCAAAACAATCCCCCGAAACGCAGGCAAAAATTGAAGACCGAGCAGAAGAAATCCG
GCGTGAAATCACACTAGCTAAAATTCGTGAGGAACTGAAACTTTCTCAAACTGAGCTAGCTCAATCACTGGGCGTTTCGC
AACCTTCTATTGCTAAGCTTGAAAATGTTGATAACGATCCGAAGCTTTCAACTCTGAAGCGCTACATTAAAGCTTTAGGC
GGTGAACTTTCTATCGATGTAACGTTACCGAATGGTAAGAGAATTGGATTGCATCTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A1H6P4A9