Detailed information of TA system TA225117

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1579118..1579745
• Replicon: chromosome
• Accession: NZ_CP087200
• Organism: Pseudomonas viciae strain B21-062

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LOY66_RS07070
• Protein ID: WP_135844147.1
• Coordinates: 1579530..1579745 (-)
• Length: 72 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LOY66_RS07065
• Protein ID: WP_256452321.1
• Coordinates: 1579118..1579528 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY66_RS07035 (LOY66_07030)	1574515..1574931	+	417	WP_135844141.1	fosfomycin resistance glutathione transferase	-
LOY66_RS07040 (LOY66_07035)	1575120..1576469	+	1350	WP_135844142.1	diguanylate cyclase	-
LOY66_RS07045 (LOY66_07040)	1576751..1577254	+	504	WP_135844143.1	DUF4142 domain-containing protein	-
LOY66_RS07050 (LOY66_07045)	1577331..1578239	+	909	WP_265072790.1	SDR family oxidoreductase	-
LOY66_RS07055 (LOY66_07050)	1578288..1578692	-	405	WP_135844145.1	low affinity iron permease family protein	-
LOY66_RS07060 (LOY66_07055)	1578866..1579012	+	147	WP_178084240.1	hypothetical protein	-
LOY66_RS07065 (LOY66_07060)	1579118..1579528	-	411	WP_256452321.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LOY66_RS07070 (LOY66_07065)	1579530..1579745	-	216	WP_135844147.1	type II toxin-antitoxin system HicA family toxin	Toxin
LOY66_RS07075 (LOY66_07070)	1580033..1580662	-	630	WP_256452323.1	LysE family translocator	-
LOY66_RS07080 (LOY66_07075)	1580808..1581287	+	480	WP_135844149.1	Lrp/AsnC family transcriptional regulator	-
LOY66_RS07085 (LOY66_07080)	1581311..1581865	-	555	WP_265072791.1	hypothetical protein	-
LOY66_RS07095 (LOY66_07090)	1582248..1583132	-	885	WP_256452325.1	alpha/beta hydrolase	-
LOY66_RS07100 (LOY66_07095)	1583442..1584133	-	692	Protein_1395	pseudouridine synthase	-
LOY66_RS07105 (LOY66_07100)	1584169..1584387	-	219	WP_135844152.1	cysteine-rich CWC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 72 a.a.
• Molecular weight: 7755.97 Da
• Isoelectric Point: 10.2823

>T225117 WP_135844147.1 NZ_CP087200:c1579745-1579530 [Pseudomonas viciae]
VNSRFLISQIIADGGYLVRVRGSHHHFKHPTKPGLVTVPHPKKDLLKKTAISILQQALLQTASCATSAEDD

Nucleotide

• Length: 216 bp

>T225117 NZ_CP120974:c3133390-3133124 [Moellerella wisconsensis]
TTGGTCTGGACGATTAATTATTCTGAACGGGCGTTAAAATCTCTACGAAAAATGGATAAACAGAGTGCTCGCCGTATTGT
CGATTTTATGGGTTTACGCATTGCAGCTTTAGATGATCCCCGACAGTTAGGCAAATCACTCAAAGGTGAACTAGGTGAGT
TCTGGCGTTATAGAGTTGGTGACTATCGGATACTATGCGAGATAAGGGATGATGAACTTGTCATTTTGGCTGCTACAATA
GGACATCGTCGTGAGATATATGAGTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14724.76 Da
• Isoelectric Point: 4.8983

>AT225117 WP_256452321.1 NZ_CP087200:c1579528-1579118 [Pseudomonas viciae]
MLYPIAISMGDDKHAWGVEVPDIPGCFSAGDDLDEAMAMAREAIEGHFEILADDGSPIPSANTVTLHAANPKYAGCTWAL
VDIDVTKYLGKAQKLNITLPGHLLNRIDEYVLHHPEEKSRSGFLASAALKVLQQGR

Nucleotide

• Length: 411 bp

>AT225117 NZ_CP120974:c3133598-3133374 [Moellerella wisconsensis]
ATGTTAGCTATTCGTTTATCTGATGACATTGAAACACGTCTGGAGTCACTTGCAAAACAGACAGGAAGAACCAAAACGTT
CTATGCGAGAGAAGCTATTCTCACCCACTTGGAAGACTTGGAAGACTACTATTTATCCGCTGAAACCATTGCAAAAATTC
GTAGCGGTGAAGAGTTGATTCATTCGTCTGAAGAGGTGAGAAAAACACTTGGTCTGGACGATTAA