Detailed information of TA system TA225111

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 4111196..4111743
• Replicon: chromosome
• Accession: NZ_CP087198
• Organism: Pseudomonas sp. B21-010

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LOY50_RS18040
• Protein ID: WP_258688768.1
• Coordinates: 4111468..4111743 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G8Q8L7
• Locus tag: LOY50_RS18035
• Protein ID: WP_014337668.1
• Coordinates: 4111196..4111468 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY50_RS18015 (LOY50_17980)	4107559..4109022	+	1464	WP_253476231.1	NADH-quinone oxidoreductase subunit NuoN	-
LOY50_RS18020 (LOY50_17985)	4109100..4109660	-	561	WP_258688767.1	TetR/AcrR family transcriptional regulator	-
LOY50_RS18025 (LOY50_17990)	4109787..4110431	+	645	WP_214512773.1	DJ-1/PfpI family protein	-
LOY50_RS18030 (LOY50_17995)	4110456..4110992	+	537	WP_214512774.1	DUF2867 domain-containing protein	-
LOY50_RS18035 (LOY50_18000)	4111196..4111468	+	273	WP_014337668.1	ribbon-helix-helix domain-containing protein	Antitoxin
LOY50_RS18040 (LOY50_18005)	4111468..4111743	+	276	WP_258688768.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOY50_RS18045 (LOY50_18010)	4111807..4112916	+	1110	WP_258688769.1	NAD(P)/FAD-dependent oxidoreductase	-
LOY50_RS18050 (LOY50_18015)	4113114..4113590	+	477	WP_003203396.1	hemerythrin domain-containing protein	-
LOY50_RS18055 (LOY50_18020)	4113647..4114525	-	879	WP_013693714.1	LysR family transcriptional regulator	-
LOY50_RS18060 (LOY50_18025)	4114771..4115400	-	630	WP_030141308.1	isochorismate family cysteine hydrolase YcaC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (10-47)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10547.11 Da
• Isoelectric Point: 9.9727

>T225111 WP_258688768.1 NZ_CP087198:4111468-4111743 [Pseudomonas sp. B21-010]
MELKWTSKALSDIARLYEFLAAVNQPAAARTVQQLTAAPTSLLANPRIGERLEEFEPRDVRRIQVGRYEMRYGIAGSTLY
LLRLWHTREDR

Nucleotide

• Length: 276 bp

>T225111 NZ_CP120971:c274958-274569 [Moellerella wisconsensis]
GTGGCGTTCTATAAAATCTCATCTTTTGTCCGAAGCACCAAAAAAATACCTATAACAGACAAACAGTTACTTGATGCTGC
TCATGAAGTTCAACAAGGACAATTTGAAGCTGATTTAGGCGGTGGAGTGATAAAAAAACGCCTTGCAATGAAAGGGCAAG
GCAAAAGTGGAAGTGTTAGGGTTATTATCTTTTTTAAGATAAACAATCATCTTTTTTTTGCAGATGGATGGACCAAAAAC
ACGGTCAATGCCAAAGGTACAAAAGAGATTGAAGATGATGAGTTGGAAACCTATAAAAAATTAGCCGCTGAGTTTCTGAA
CTTTGATGGAGAAATCATTAACAATTTAATTTCTAAAGGAATTCTGGAGGAAATTACTGATGAACAATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10108.40 Da
• Isoelectric Point: 6.0741

>AT225111 WP_014337668.1 NZ_CP087198:4111196-4111468 [Pseudomonas sp. B21-010]
MTTTAKTRSVTAHVPVHLAEKVDLMAERLERSRNWIVKQALSAWIEQEEERSRLTHEALADVDAGRVIDHQAVQAWADSL
STDAPLPVPR

Nucleotide

• Length: 273 bp

>AT225111 NZ_CP120971:c274579-274250 [Moellerella wisconsensis]
ATGAACAATAAACTTAATAACATGCTTGAATTGGCTAAAGAGTTAAATGCAGCTGGTGCATCACCTGAAAGCCTGGTTAA
AGATATTGAAAGCACCATCCGTGCTCGTGAAATTAACGATAAAATAAAAGAAGTTTCAGCGATGAGCGGCTCTGAGATCA
AGATGTTGCGGACTAAATATTGTATGTCTCAATCTGTGCTCGCTCGTGCATTGGGAATGTCGAAAGAGAGCATATCCAAA
TGGGAGCGAGGAGAAAAGAAACCAAGCGGCCCTGCATTGAGGATGCTACGTATTCTTGAAAGCAAAGGCCCAGAAGTTTT
AGTGGTCTAA

Structures

Antitoxin

• AlphaFold DB: G8Q8L7