Detailed information of TA system TA225110

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 2442959..2443478
• Replicon: chromosome
• Accession: NZ_CP087198
• Organism: Pseudomonas sp. B21-010

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LOY50_RS10890
• Protein ID: WP_186650661.1
• Coordinates: 2443197..2443478 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A176NDU7
• Locus tag: LOY50_RS10885
• Protein ID: WP_024616638.1
• Coordinates: 2442959..2443207 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY50_RS10870 (LOY50_10840)	2439387..2440385	+	999	WP_258691222.1	AAA family ATPase	-
LOY50_RS10875 (LOY50_10845)	2440487..2441044	+	558	WP_258690826.1	VWA domain-containing protein	-
LOY50_RS10880 (LOY50_10850)	2441246..2442892	+	1647	WP_258690827.1	phospholipase D-like domain-containing protein	-
LOY50_RS10885 (LOY50_10855)	2442959..2443207	+	249	WP_024616638.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LOY50_RS10890 (LOY50_10860)	2443197..2443478	+	282	WP_186650661.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOY50_RS10895 (LOY50_10865)	2443593..2443934	+	342	WP_253473429.1	hypothetical protein	-
LOY50_RS10900 (LOY50_10870)	2444324..2445172	+	849	WP_003200222.1	transporter substrate-binding domain-containing protein	-
LOY50_RS10905 (LOY50_10875)	2445227..2445889	+	663	WP_014338685.1	amino acid ABC transporter permease	-
LOY50_RS10910 (LOY50_10880)	2445898..2446560	+	663	WP_014338686.1	amino acid ABC transporter permease	-
LOY50_RS10915 (LOY50_10885)	2446557..2447363	+	807	WP_253473435.1	amino acid ABC transporter ATP-binding protein	-
LOY50_RS10920 (LOY50_10890)	2447390..2447779	+	390	WP_013692714.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10889.62 Da
• Isoelectric Point: 10.1953

>T225110 WP_186650661.1 NZ_CP087198:2443197-2443478 [Pseudomonas sp. B21-010]
MTYELEFSDKAWKEWQKLGATLKEQFKKKLEERLVNPHVQADRLHGLGNAYKIKLRNAGYRLVYRVVDDLLVVTVIAVGK
RERGQVYDEAAKR

Nucleotide

• Length: 282 bp

>T225110 NZ_CP120971:c130241-129909 [Moellerella wisconsensis]
ATGGTATCTCGATACGTACCTGATTCTGGCGATTTGATTTGGATTGACTTTGATCCAGTTGAAGGACATGAGCAGGGCGG
TCATCGTCCAGCAGTTGTCCTTAGCCCTTTTGCATACAATAATAAAGTAGGGCTCTTACTATGCGTACCATGTACAACTA
AAGGCAAAGGCTATCCATTTGAATGCGAACTCTCGAACGAAAGAGACAGCTACGCATTAGCGGATCAAGTTACATGTGTT
GACTGGAGAGCTCGTAAAGTTACTAAAAAGGGCAAAGTGGAGGCATCAGAACTGGCAGAGATAAAAGCTAAAGCAAAGGC
GTTAATAGGATAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9037.44 Da
• Isoelectric Point: 4.4000

>AT225110 WP_024616638.1 NZ_CP087198:2442959-2443207 [Pseudomonas sp. B21-010]
MTHIVLSQVVASISELKKNPMGTVAAGGGLSVAILNRNEPAFYCVPAREYEAMMDRLEDMELVALCRERENDPTIKVSLD
DL

Nucleotide

• Length: 249 bp

>AT225110 NZ_CP120971:c130486-130241 [Moellerella wisconsensis]
ATGGCTCAGGTCACAGTAAAAAAATGGGGCAATAGCCCGTCTGTACGGCTTCCTGTTGCTATCATGCAAAAAGCGGCACT
TAGCGTTGATGATACGGTTGAAATAGCGGTAGAAGAAGGTCGTATCATCATTACGCCAGTTAAAGCGGTAGAATACTCAT
TAGATAATTTGTTAGCCGGTATCACACCGGAGAATATCCATGAGAAAGTAGACTTCGGAGCTCGAACAGGTAAGGAATTA
ATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A176NDU7