Detailed information of TA system TA225108

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 330805..331481
• Replicon: chromosome
• Accession: NZ_CP087198
• Organism: Pseudomonas sp. B21-010

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1H4VVN5
• Locus tag: LOY50_RS01420
• Protein ID: WP_018608816.1
• Coordinates: 330805..330987 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LOY50_RS01425
• Protein ID: WP_024619603.1
• Coordinates: 331080..331481 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY50_RS01405 (LOY50_01400)	326800..328761	+	1962	WP_253478360.1	choline transporter BetT	-
LOY50_RS01410 (LOY50_01405)	328928..329668	-	741	WP_003196671.1	SDR family oxidoreductase	-
LOY50_RS01415 (LOY50_01410)	329785..330675	+	891	WP_258690175.1	LysR family transcriptional regulator	-
LOY50_RS01420 (LOY50_01415)	330805..330987	+	183	WP_018608816.1	type II toxin-antitoxin system HicA family toxin	Toxin
LOY50_RS01425 (LOY50_01420)	331080..331481	+	402	WP_024619603.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LOY50_RS01430 (LOY50_01425)	331540..332577	+	1038	WP_258690176.1	L-glyceraldehyde 3-phosphate reductase	-
LOY50_RS01435 (LOY50_01430)	332805..333647	-	843	WP_258690177.1	taurine dioxygenase	-
LOY50_RS01440 (LOY50_01435)	333698..334537	-	840	WP_214510990.1	taurine ABC transporter permease TauC	-
LOY50_RS01445 (LOY50_01440)	334534..335328	-	795	WP_095963000.1	taurine ABC transporter ATP-binding subunit	-
LOY50_RS01450 (LOY50_01445)	335350..336327	-	978	WP_253470920.1	taurine ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6898.03 Da
• Isoelectric Point: 10.7459

>T225108 WP_018608816.1 NZ_CP087198:330805-330987 [Pseudomonas sp. B21-010]
MRSREMIRKIEEDGWYLVAIKGSHHQYKHAFKAGRVTIKHPDADLPRGTINSILKQAGLK

Nucleotide

• Length: 183 bp

>T225108 NZ_CP120970:c3133529-3133263 [Moellerella wisconsensis]
TTGGTCTGGACGATTAATTATTCTGAACGGGCGTTAAAATCTCTACGAAAAATGGATAAACAGAGTGCTCGCCGTATTGT
CGATTTTATGGGTTTACGCATTGCAGCTTTAGATGATCCCCGACAGTTAGGCAAATCACTCAAAGGTGAACTAGGTGAGT
TCTGGCGTTATAGAGTTGGTGACTATCGGATACTATGCGAGATAAGGGATGATGAACTTGTCATTTTGGCTGCTACAATA
GGACATCGTCGTGAGATATATGAGTAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14683.38 Da
• Isoelectric Point: 4.4085

>AT225108 WP_024619603.1 NZ_CP087198:331080-331481 [Pseudomonas sp. B21-010]
MKFPVVLHKDADSEYGVTVPDVPGCFSAGSTVSQAFENVKEALSLHYEGLIADGEPLPQIHEIDDHLDDPQYAGGIWGVV
EFDVTPYFGKSVRFNATLPEQLLERIDQTVRRDQRYSSRSGFLAAAALRELSA

Nucleotide

• Length: 402 bp

>AT225108 NZ_CP120970:c3133737-3133513 [Moellerella wisconsensis]
ATGTTAGCTATTCGTTTATCTGATGACATTGAAACACGTCTGGAGTCACTTGCAAAACAGACAGGAAGAACCAAAACGTT
CTATGCGAGAGAAGCTATTCTCACCCACTTGGAAGACTTGGAAGACTACTATTTATCCGCTGAAACCATTGCAAAAATTC
GTAGCGGTGAAGAGTTGATTCATTCGTCTGAAGAGGTGAGAAAAACACTTGGTCTGGACGATTAA

Structures

Toxin

• AlphaFold DB: A0A1H4VVN5