Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | PumAB/upstrm_HI1419-dnstrm_HI1420 |
| Location | 4624741..4625342 | Replicon | chromosome |
| Accession | NZ_CP087195 | ||
| Organism | Pseudomonas brassicacearum strain B21-016 | ||
Toxin (Protein)
| Gene name | PumA | Uniprot ID | - |
| Locus tag | LOY47_RS20300 | Protein ID | WP_198286652.1 |
| Coordinates | 4624741..4625031 (+) | Length | 97 a.a. |
Antitoxin (Protein)
| Gene name | PumB | Uniprot ID | A0A1G4U731 |
| Locus tag | LOY47_RS20305 | Protein ID | WP_038858737.1 |
| Coordinates | 4625034..4625342 (+) | Length | 103 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LOY47_RS20265 (LOY47_20250) | 4620224..4620661 | - | 438 | WP_233839792.1 | GNAT family N-acetyltransferase | - |
| LOY47_RS20270 (LOY47_20255) | 4620725..4621231 | - | 507 | WP_100264118.1 | AAA family ATPase | - |
| LOY47_RS20275 (LOY47_20260) | 4621532..4621732 | - | 201 | WP_013693816.1 | hypothetical protein | - |
| LOY47_RS20280 (LOY47_20265) | 4621838..4622263 | - | 426 | WP_003203795.1 | hypothetical protein | - |
| LOY47_RS20285 (LOY47_20270) | 4622517..4622735 | - | 219 | WP_043042785.1 | hypothetical protein | - |
| LOY47_RS20290 (LOY47_20275) | 4622797..4623342 | - | 546 | WP_043042784.1 | DUF4410 domain-containing protein | - |
| LOY47_RS20295 (LOY47_20280) | 4624044..4624469 | + | 426 | WP_258636024.1 | hypothetical protein | - |
| LOY47_RS20300 (LOY47_20285) | 4624741..4625031 | + | 291 | WP_198286652.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| LOY47_RS20305 (LOY47_20290) | 4625034..4625342 | + | 309 | WP_038858737.1 | putative addiction module antidote protein | Antitoxin |
| LOY47_RS20310 (LOY47_20295) | 4625469..4625741 | - | 273 | WP_003203821.1 | hypothetical protein | - |
| LOY47_RS20315 (LOY47_20300) | 4626394..4626726 | + | 333 | WP_218180050.1 | transposase | - |
| LOY47_RS20320 (LOY47_20305) | 4626723..4627058 | + | 336 | WP_258636028.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| LOY47_RS20325 (LOY47_20310) | 4627140..4628708 | + | 1569 | WP_258636035.1 | IS66 family transposase | - |
| LOY47_RS20330 (LOY47_20315) | 4629251..4629748 | - | 498 | WP_258636037.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | - | 4615520..4636722 | 21202 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 97 a.a. Molecular weight: 10502.01 Da Isoelectric Point: 10.8582
>T225100 WP_198286652.1 NZ_CP087195:4624741-4625031 [Pseudomonas brassicacearum]
IYRIQQTQTFTAWHSSVRDLRAKNAIARRIDRAAAGNLGDVKPVGDGISELRVDVGAGYRDYFTMRGGLVIVLLAGGDKS
SQSADIRRAKKLAKEI
IYRIQQTQTFTAWHSSVRDLRAKNAIARRIDRAAAGNLGDVKPVGDGISELRVDVGAGYRDYFTMRGGLVIVLLAGGDKS
SQSADIRRAKKLAKEI
Download Length: 291 bp
>T225100 NZ_CP120969:930967-931344 [Pseudomonas putida]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGTGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGTGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA
Antitoxin
Download Length: 103 a.a. Molecular weight: 10928.34 Da Isoelectric Point: 5.9574
>AT225100 WP_038858737.1 NZ_CP087195:4625034-4625342 [Pseudomonas brassicacearum]
MSQHLTTFDMAALLDSDEAISEYLSQVLADGDSEEFLRAIGYVAKARGMAQIAKDSGMGRESLYKAFAPGSKPRFDTVLK
VIHALGIDLHAQPGHAANHSPA
MSQHLTTFDMAALLDSDEAISEYLSQVLADGDSEEFLRAIGYVAKARGMAQIAKDSGMGRESLYKAFAPGSKPRFDTVLK
VIHALGIDLHAQPGHAANHSPA
Download Length: 309 bp
>AT225100 NZ_CP120969:931325-931660 [Pseudomonas putida]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|