Detailed information of TA system TA225099

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 397767..398286
• Replicon: chromosome
• Accession: NZ_CP087195
• Organism: Pseudomonas brassicacearum strain B21-016

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LOY47_RS01740
• Protein ID: WP_027911783.1
• Coordinates: 397996..398286 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0B1YWZ8
• Locus tag: LOY47_RS01735
• Protein ID: WP_027911784.1
• Coordinates: 397767..398006 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY47_RS01710 (LOY47_01710)	394153..395076	-	924	WP_013692056.1	LysR family transcriptional regulator	-
LOY47_RS01715 (LOY47_01715)	395192..395956	+	765	WP_013692057.1	SDR family oxidoreductase	-
LOY47_RS01720 (LOY47_01720)	396176..396428	+	253	Protein_336	type II toxin-antitoxin system HicA family toxin	-
LOY47_RS01725 (LOY47_01725)	396425..396757	+	333	WP_003196781.1	type II toxin-antitoxin system HicB family antitoxin	-
LOY47_RS01730 (LOY47_01730)	396922..397200	-	279	WP_027911785.1	DUF3077 domain-containing protein	-
LOY47_RS01735 (LOY47_01735)	397767..398006	+	240	WP_027911784.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LOY47_RS01740 (LOY47_01740)	397996..398286	+	291	WP_027911783.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOY47_RS01745 (LOY47_01745)	398688..399602	-	915	WP_053161729.1	FRG domain-containing protein	-
LOY47_RS01750 (LOY47_01750)	399879..401504	-	1626	WP_258636529.1	TIGR04141 family sporadically distributed protein	-
LOY47_RS01755 (LOY47_01755)	402894..403223	-	330	WP_053161734.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11305.13 Da
• Isoelectric Point: 10.4047

>T225099 WP_027911783.1 NZ_CP087195:397996-398286 [Pseudomonas brassicacearum]
MTYSLEFDARALKEWRKLGNTVREQFKKKLTTILANPRIEANRLHAFPDCYKIKLRSSGYRLVYQVIDQEVVVFVVAVDK
RERDQVYRKAAERLGG

Nucleotide

• Length: 291 bp

>T225099 NZ_CP120969:920028-920405 [Pseudomonas putida]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGTGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8968.36 Da
• Isoelectric Point: 4.8011

>AT225099 WP_027911784.1 NZ_CP087195:397767-398006 [Pseudomonas brassicacearum]
MASINVRIDDDLKARAYRELEKLGVTPSELMRQALQYVAERGQLPFKPVLMTEDDEALIATVRERLAAPQRVKVSLDDL

Nucleotide

• Length: 240 bp

>AT225099 NZ_CP120969:920386-920721 [Pseudomonas putida]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0B1YWZ8