Detailed information of TA system TA225097

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 4797431..4798470
• Replicon: chromosome
• Accession: NZ_CP087194
• Organism: Pseudomonas sp. B21-017

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: LOY28_RS21580
• Protein ID: WP_064619432.1
• Coordinates: 4797895..4798470 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A2S8HVM2
• Locus tag: LOY28_RS21575
• Protein ID: WP_018925078.1
• Coordinates: 4797431..4797898 (+)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY28_RS21555 (LOY28_21530)	4793126..4793950	+	825	WP_258612517.1	ion transporter	-
LOY28_RS21560 (LOY28_21535)	4794057..4795055	+	999	WP_018925081.1	sulfate ABC transporter substrate-binding protein	-
LOY28_RS21565 (LOY28_21540)	4795113..4795745	-	633	WP_258612518.1	hypothetical protein	-
LOY28_RS21570 (LOY28_21545)	4795981..4797186	+	1206	WP_258612519.1	MFS transporter	-
LOY28_RS21575 (LOY28_21550)	4797431..4797898	+	468	WP_018925078.1	helix-turn-helix domain-containing protein	Antitoxin
LOY28_RS21580 (LOY28_21555)	4797895..4798470	+	576	WP_064619432.1	PIN domain-containing protein	Toxin
LOY28_RS21585 (LOY28_21560)	4798527..4798925	-	399	WP_086945855.1	hypothetical protein	-
LOY28_RS21590 (LOY28_21565)	4798940..4800148	-	1209	WP_258612520.1	c-type cytochrome biogenesis protein CcmI	-
LOY28_RS21595 (LOY28_21570)	4800141..4800614	-	474	WP_018925074.1	cytochrome c-type biogenesis protein CcmH	-
LOY28_RS21600 (LOY28_21575)	4800611..4801147	-	537	WP_018925073.1	DsbE family thiol:disulfide interchange protein	-
LOY28_RS21605 (LOY28_21580)	4801144..4803147	-	2004	WP_018925072.1	heme lyase CcmF/NrfE family subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21765.90 Da
• Isoelectric Point: 5.6166

>T225097 WP_064619432.1 NZ_CP087194:4797895-4798470 [Pseudomonas sp. B21-017]
VRHSPFTAVYDACVLYPAPLRDFLMWLALSGRFRARWSLEIHNEWKRNLLKNRPDLTTEQLDRTSDLMDQAIPDACVYGY
ERLIEGLTLPDVDDRHVLAAAIRCNASVIVTFNQKDFPDETLGPFGIEAQHPDEFVDNLFDLDPAAVVAAAQRQRKQLKT
PPMDVSTYLELLLRLGLTRSSKALTHYQAIL

Nucleotide

• Length: 576 bp

>T225097 NZ_CP120967:c19199-18855 [Lactiplantibacillus plantarum]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAAGCGACCAGCCAT
TGCGCTAAGCAATGATTTAGTCTGTCAGACGAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACGGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTAGATTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17303.72 Da
• Isoelectric Point: 5.5294

>AT225097 WP_018925078.1 NZ_CP087194:4797431-4797898 [Pseudomonas sp. B21-017]
MSPVIHPPLNLPMEREVQAAIEGQRALAAYLATQFETQHIQIFDEQNEAHSVELPTSALRLLVDILAELAAGNAVKVVPV
HAELTTQEAADLLNVSRPHLIKLLESGELSYHKTGKHRRVRFTDLMDYKTQRDIASEQAMTLLAEQAQALRTGYE

Nucleotide

• Length: 468 bp

>AT225097 NZ_CP120967:c19456-19193 [Lactiplantibacillus plantarum]
ATGGAACTTATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAACTATTTAAGGATTGGCAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2S8HVM2