Detailed information of TA system TA225094

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-YefM
• Location: 1109526..1110031
• Replicon: chromosome
• Accession: NZ_CP087194
• Organism: Pseudomonas sp. B21-017

Toxin (Protein)

• Gene name: relE
• Uniprot ID: J2N6H1
• Locus tag: LOY28_RS04840
• Protein ID: WP_007940443.1
• Coordinates: 1109526..1109780 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: LOY28_RS04845
• Protein ID: WP_007940444.1
• Coordinates: 1109777..1110031 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY28_RS04820 (LOY28_04820)	1105515..1106231	+	717	WP_064616221.1	peptide-methionine (S)-S-oxide reductase MsrA	-
LOY28_RS04825 (LOY28_04825)	1106247..1107260	-	1014	WP_018930167.1	sensor domain-containing diguanylate cyclase	-
LOY28_RS04830 (LOY28_04830)	1107336..1108241	-	906	WP_258613147.1	LysR family transcriptional regulator	-
LOY28_RS04835 (LOY28_04835)	1108344..1109519	+	1176	WP_258613148.1	aspartate aminotransferase family protein	-
LOY28_RS04840 (LOY28_04840)	1109526..1109780	-	255	WP_007940443.1	Txe/YoeB family addiction module toxin	Toxin
LOY28_RS04845 (LOY28_04845)	1109777..1110031	-	255	WP_007940444.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LOY28_RS04850 (LOY28_04850)	1110259..1110786	-	528	WP_018930170.1	adenylate kinase	-
LOY28_RS04855 (LOY28_04855)	1110803..1112740	-	1938	WP_258613149.1	alkaline phosphatase D family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-71)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9997.52 Da
• Isoelectric Point: 8.0472

>T225094 WP_007940443.1 NZ_CP087194:c1109780-1109526 [Pseudomonas sp. B21-017]
MNIEFTPEGWDDYLWFQQNDKAGLKRINLLIKAILREPFEGLGKPEPLKHNLSGLWSRRITSEHRLVYAIENGEVRIVLC
RYHY

Nucleotide

• Length: 255 bp

>T225094 NZ_CP120956:5867765-5868256 [Delftia tsuruhatensis]
ATGAGCGCCGTACCGCCCGCCCCCTTGCGCATCCACGGCTGGACGGTATTCATGCACCCGCTTTTCCTGGCCCAGCTACA
GGCGTTGAGCCACGAAGTGGAGGGTCTGAAAACCAAGGACCCTGGCGGGTACACACGCAAGAACGCGACCAAGCGATTGG
CGGCGATCCGCAAGCTGGCATTCGATGTCATCCCGCAGGATCCGACGCGGCCAGACTACCGCCAGGGCCATACCCTGGGC
GAGGAGCACAAGCACTGGTTTCGGGCCAGGTTCTTCCAGCAGTACCGCCTGTTCTTTCGCTTTCACGCCCCCAGCAAAAT
CATCGTGTTCGCCTGGGTCAACGATGAGGACACCAAGCGAGCCTATGAGGGCAGCGACGATGCCTACAGGGTGTTCCGAA
AAATGCTCGCCAACGGGCACCCTCCTGGCGATTGGGACAGTTTGCTGGCACAGGCGAAGTCCCCGCCACCCGACTCACAG
CCGCACGCCTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9500.94 Da
• Isoelectric Point: 8.5163

>AT225094 WP_007940444.1 NZ_CP087194:c1110031-1109777 [Pseudomonas sp. B21-017]
MQTINYTTARAHLAETMDRVNEDRAPLLVTRQKGEPVVMMSLAEYNALEETAYLLRSPANAERLIKSIGNLRAGKTKVRQ
LIEE

Nucleotide

• Length: 255 bp

>AT225094 NZ_CP120956:5867439-5867768 [Delftia tsuruhatensis]
ATGTTTTCGACACTTGAAGCCGAATCCACGCTCACTGACCGCTACCAGACCACCATTCCCGAAGCCGTTCGCCGCGCATT
GCACCTGAGCAAGCGCGACAGGATCCACTACACCATCCTGCCCGGCGGGGAGGTCGTGCTCAGTCGCGCCGACTCCACCG
AGGATGCCGATCCCGTGCTGGGCCAGTTTCTCGGCTTTCTGGCCCAGGACATGGCAGCCCACCCCGAGCGCCTGCAGACC
ATGGATGCCCGTCTGGCGCAGCAGATCCAGGCGCTGACCGAAGGCATCGAGGTCGATCTGGATGCACCGCTTCTGGCAGA
CGACGAATGA

Structures

Toxin

• AlphaFold DB: J2N6H1