Detailed information of TA system TA225092

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 4584240..4584856
• Replicon: chromosome
• Accession: NZ_CP087193
• Organism: Pseudomonas sp. B21-019

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A291AEE6
• Locus tag: LOY36_RS20325
• Protein ID: WP_019579596.1
• Coordinates: 4584644..4584856 (-)
• Length: 71 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LOY36_RS20320
• Protein ID: WP_258670509.1
• Coordinates: 4584240..4584644 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY36_RS20300 (LOY36_20275)	4580077..4580901	+	825	WP_019648131.1	ion transporter	-
LOY36_RS20305 (LOY36_20280)	4581008..4582006	+	999	WP_019648130.1	sulfate ABC transporter substrate-binding protein	-
LOY36_RS20310 (LOY36_20285)	4582066..4582698	-	633	WP_258670503.1	hypothetical protein	-
LOY36_RS20315 (LOY36_20290)	4582935..4584140	+	1206	WP_258670506.1	MFS transporter	-
LOY36_RS20320 (LOY36_20295)	4584240..4584644	-	405	WP_258670509.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LOY36_RS20325 (LOY36_20300)	4584644..4584856	-	213	WP_019579596.1	type II toxin-antitoxin system HicA family toxin	Toxin
LOY36_RS20330 (LOY36_20305)	4585172..4585552	-	381	WP_258670514.1	hypothetical protein	-
LOY36_RS20335 (LOY36_20310)	4585567..4586775	-	1209	WP_258670516.1	c-type cytochrome biogenesis protein CcmI	-
LOY36_RS20340 (LOY36_20315)	4586768..4587241	-	474	WP_018925074.1	cytochrome c-type biogenesis protein CcmH	-
LOY36_RS20345 (LOY36_20320)	4587238..4587774	-	537	WP_258670519.1	DsbE family thiol:disulfide interchange protein	-
LOY36_RS20350 (LOY36_20325)	4587771..4589774	-	2004	WP_258670522.1	heme lyase CcmF/NrfE family subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7834.11 Da
• Isoelectric Point: 10.3517

>T225092 WP_019579596.1 NZ_CP087193:c4584856-4584644 [Pseudomonas sp. B21-019]
VQSRLLIKELMEAGWTLDRVTGSHHIFTHRYNPYTIPVPHPKKDLPIGTVKSIRRRAGLYCPGASLAGDP

Nucleotide

• Length: 213 bp

>T225092 NZ_CP120956:4309674-4310120 [Delftia tsuruhatensis]
ATGACACGCTACCTGCTGGACACCAACATCGCCAGCCACATCATCAAAGGCGATATCGCTGCCGTGCGCGAACAGCTGGT
TCGCGTACCCATGCACCAGATCGCAGTGTCGGCGGTCACGCAGGCCGAACTGATGTACGGGGTCGCCAAGCGCGGCCACC
CTGCCGGGCTGTCGCTGCGGGTGACAGGGTTCCTGGCCCGCGTCGAGGTGCTGCCCTGGACGGCCCAGGTGGCCGATGCC
TACGGCCACCTGCGCGCCGCCTGCGAGGCGGCCGGCATCACGCTGGCCTCCATGGACATGATGATTGCCGCGCACGCCCT
GCTCCTGCAGCAGCAGGCCGCGCAGGCCGGCGAGCGCTCGGTGCTGGTCACAAGAGACCGGGTGTTCAGCCGCATTCCCG
AACCCGGACTGGCCATCGCGGACTGGACCACCGGCCCGGCTGGCTGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14887.94 Da
• Isoelectric Point: 4.4268

>AT225092 WP_258670509.1 NZ_CP087193:c4584644-4584240 [Pseudomonas sp. B21-019]
MQYPICIEWGDENTAIGIQIPDIPGAVTAGDTFEDAYNAAVEIAHIMLQEIMADGESIPMPTSAAAHRENPEFADMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHKVKSRSSFLADAALEKLIRH

Nucleotide

• Length: 405 bp

>AT225092 NZ_CP120956:4309411-4309677 [Delftia tsuruhatensis]
GTGACGCAGACTGCCAAGCTTTTCACCACCGGTCGCAGCCAGGCCGTGCGCCTTCCCTATGAATTCCGGTTCGAGGAAAA
AGAGGTCTACATACGTCGCGACCCCGTCACGGGCGACGTGATCCTGTCGCGCCGTCCCGACTCATGGCAAGAGTTCTTTG
CGCTCGACGCCACCACCGACGTGCCGGCCGATTTCATGGACACCGCTGACCGAGCGCAGCCAGAGTCAGGCCGCGATCCG
TTTGCCGACGAAGGCAGTGCGCGATGA

Structures

Toxin

• AlphaFold DB: A0A291AEE6