Detailed information of TA system TA225090

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 2228502..2229002
• Replicon: chromosome
• Accession: NZ_CP087193
• Organism: Pseudomonas sp. B21-019

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LOY36_RS10025
• Protein ID: WP_258673173.1
• Coordinates: 2228814..2229002 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: LOY36_RS10020
• Protein ID: WP_258673171.1
• Coordinates: 2228502..2228675 (+)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY36_RS09975 (LOY36_09970)	2223825..2224121	-	297	WP_258673149.1	hypothetical protein	-
LOY36_RS09980 (LOY36_09975)	2224291..2224797	-	507	WP_258673151.1	hypothetical protein	-
LOY36_RS09985 (LOY36_09980)	2225816..2226412	+	597	WP_258673153.1	recombinase family protein	-
LOY36_RS09990 (LOY36_09985)	2226616..2226753	+	138	WP_258673155.1	hypothetical protein	-
LOY36_RS09995 (LOY36_09990)	2226956..2227174	+	219	WP_217857771.1	ribbon-helix-helix protein, CopG family	-
LOY36_RS10000 (LOY36_09995)	2227317..2227559	+	243	WP_258673167.1	hypothetical protein	-
LOY36_RS10005 (LOY36_10000)	2227598..2227816	+	219	WP_217857770.1	CopG family transcriptional regulator	-
LOY36_RS10010 (LOY36_10005)	2227939..2228081	+	143	Protein_1959	transcriptional regulator	-
LOY36_RS10015 (LOY36_10010)	2228267..2228458	+	192	WP_258673170.1	hypothetical protein	-
LOY36_RS10020 (LOY36_10015)	2228502..2228675	+	174	WP_258673171.1	hypothetical protein	Antitoxin
LOY36_RS10025 (LOY36_10020)	2228814..2229002	+	189	WP_258673173.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LOY36_RS10030 (LOY36_10025)	2230118..2230825	-	708	WP_258673183.1	type I-E CRISPR-associated protein Cas5/CasD	-
LOY36_RS10035 (LOY36_10030)	2230829..2231848	-	1020	WP_258673196.1	type I-E CRISPR-associated protein Cas7/Cse4/CasC	-
LOY36_RS10040 (LOY36_10035)	2231851..2232486	-	636	WP_258673198.1	type I-E CRISPR-associated protein Cas6/Cse3/CasE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2227317..2267974	40657

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-58)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6746.02 Da
• Isoelectric Point: 10.7531

>T225090 WP_258673173.1 NZ_CP087193:2228814-2229002 [Pseudomonas sp. B21-019]
MTTQIKGYPFKVPIAGAKPSAVLADKVKRVDWVARKVQYKGQLTAAELEQIQRVVAALIGPP

Nucleotide

• Length: 189 bp

>T225090 NZ_CP120956:338778-339059 [Delftia tsuruhatensis]
ATGACGATCCAGTCATTCAAGTGCCCCGACACCGAGCAACTGTTCCAGCAGAGGCGGGTGCGTCGGTGGAGCAGCATTGA
AGTGGTTGCGCTGCGCAAGCTTCGGATGCTGCATGCCGCTCATGTGCTTCAGGACTTGAGGGTCCCGCCCGGAAACCGGC
TGGAGGCGCTGCACGGAGATCGCAAGGGGCAGCACAGCATCCGCATCAATGACCAGTGGCGCGTGTGCTTTGTGTGGACC
CATGAAGGCCCGAGTGAGCTGGAGATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6565.44 Da
• Isoelectric Point: 5.6580

>AT225090 WP_258673171.1 NZ_CP087193:2228502-2228675 [Pseudomonas sp. B21-019]
VFELDLEVDVRVEGNRMIIEPVRESLDLARLLAKVTPENRHEAVDWGHRLARRSGNS

Nucleotide

• Length: 174 bp

>AT225090 NZ_CP120956:339102-339401 [Delftia tsuruhatensis]
ATGAACAAACCTTTGAACCTCCCCACGCCAGGCGAAATCCTGGCCACTGAATTCCTGGAGCCCATGGGCCTGACGCAATA
CCGCCTGGCCCAGGCCATCCATGTCCCGCAGACACGCATCGCGGCCATCGTCAAGAACGGCCGCTCCATCACCGCGGATA
CGGCGCTGCGCCTTGCGCGCTTCTTTGGCACGTCCGTGGAGTTCTGGATGAATCTGCAAAGCCAGTACGACGCCGATATC
GCGCGCGAGGCCATAGGCGACGACCTGGAGTCCATCGAGCCCTTTGCTCTGCAGGCTTGA