Detailed information of TA system TA225088

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1807109..1808148
• Replicon: chromosome
• Accession: NZ_CP087192
• Organism: Pseudomonas sp. B21-021

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: LOY31_RS08125
• Protein ID: WP_221534218.1
• Coordinates: 1807109..1807684 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: LOY31_RS08130
• Protein ID: WP_221534216.1
• Coordinates: 1807681..1808148 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY31_RS08100 (LOY31_08090)	1802436..1804424	+	1989	WP_258713090.1	heme lyase CcmF/NrfE family subunit	-
LOY31_RS08105 (LOY31_08095)	1804421..1804957	+	537	WP_085611514.1	DsbE family thiol:disulfide interchange protein	-
LOY31_RS08110 (LOY31_08100)	1804954..1805424	+	471	WP_221534220.1	cytochrome c-type biogenesis protein CcmH	-
LOY31_RS08115 (LOY31_08105)	1805421..1806623	+	1203	WP_221534219.1	c-type cytochrome biogenesis protein CcmI	-
LOY31_RS08120 (LOY31_08110)	1806649..1807056	+	408	WP_085605822.1	hypothetical protein	-
LOY31_RS08125 (LOY31_08115)	1807109..1807684	-	576	WP_221534218.1	PIN domain-containing protein	Toxin
LOY31_RS08130 (LOY31_08120)	1807681..1808148	-	468	WP_221534216.1	helix-turn-helix domain-containing protein	Antitoxin
LOY31_RS08135 (LOY31_08125)	1808234..1808326	-	93	Protein_1602	LysR family transcriptional regulator	-
LOY31_RS08140 (LOY31_08130)	1808429..1808833	+	405	WP_085666700.1	type II toxin-antitoxin system HicB family antitoxin	-
LOY31_RS08145 (LOY31_08135)	1808879..1810066	-	1188	WP_258713091.1	MFS transporter	-
LOY31_RS08150 (LOY31_08140)	1810308..1810928	+	621	WP_258713092.1	DUF5666 domain-containing protein	-
LOY31_RS08155 (LOY31_08145)	1810984..1811982	-	999	WP_221534212.1	sulfate ABC transporter substrate-binding protein	-
LOY31_RS08160 (LOY31_08150)	1812089..1812913	-	825	WP_258713093.1	ion transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21602.94 Da
• Isoelectric Point: 6.4807

>T225088 WP_221534218.1 NZ_CP087192:c1807684-1807109 [Pseudomonas sp. B21-021]
VRHSSFTALYDACVLYPAPLRDFLMWLALSGRFRARWTQEIHCEWKRNLLKNRRDLTMEQLDRTSGLMDQAIPDACVHDY
EDLIAGLSLPDCDDRHVLAAAIRCGASVIVTFNQKDFPCKALEPFGIEAQHPDEFVDNLLGLDPAAVVAAAQKQRRQLKM
PAMDVEPFLDLLQRQGLIKSVRALSGYHAIL

Nucleotide

• Length: 576 bp

>T225088 NZ_CP120953:c1946610-1946356 [Streptococcus agalactiae]
ATGTTTAATTTTACTGAAGAAGCATGGAAAGATTATGTTTCTTGGCAACAGGAAGATAAAAAAATCTTGAAACGAATTAA
TCGTTTGATAGAAGATATTAAAAGAGACCCATTTGAAGGCATTGGAAAACCAGAACCTTTAAAATACCATTATTCAGGGG
CTTGGTCTCGAAGAATTACAGAAGAACATAGGTTGATTTATATGATAGAAGATGGAGAAATCTATTTTTTATCTTTTAGA
GATCATTATAAGTAG

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17286.73 Da
• Isoelectric Point: 6.4965

>AT225088 WP_221534216.1 NZ_CP087192:c1808148-1807681 [Pseudomonas sp. B21-021]
MSTVIHPSISLPVEREVKAAIEGQRALAAYLATHFETQHIQIFDEQNEAHRVELPTSALRLLIDILAELAAGNAVQVVPV
HAELTTQEAADLLNVSRPHLIKLLESGELSYHKTGKHRRVRFADLMEYKTRRTTASEQAMALLAEQAQALRTGYE

Nucleotide

• Length: 468 bp

>AT225088 NZ_CP120953:c1946869-1946603 [Streptococcus agalactiae]
ATGGAGGCTATTGTTTATTCACATTTTAGAAATAATTTAAAAGATTATATGAAGAAGGTCAATGATGAATTTGAGCCTTT
GATTGTAGTTAATAAAAATCCTGATGAAAATATAGTGGTTTTATCTCAAGATAGCTGGGAAAGTTTACAAGAAACGATTC
GTTTGATGGAAAATGATTATCTATCACATAAGGTTATTAATGGTATATCACAAGTTAAAGAGAAGCAAGTCACTAAACAT
GGTTTAATTGAGGTAGAAGATGTTTAA