Detailed information of TA system TA225086

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1437372..1439065
• Replicon: chromosome
• Accession: NZ_CP087191
• Organism: Pseudomonas wadenswilerensis strain B21-022

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: LOY45_RS06645
• Protein ID: WP_233673369.1
• Coordinates: 1438196..1439065 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: LOY45_RS06640
• Protein ID: WP_115088231.1
• Coordinates: 1437372..1438199 (-)
• Length: 276 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY45_RS06620 (LOY45_06615)	1432580..1434070	-	1491	WP_115088235.1	aldehyde dehydrogenase	-
LOY45_RS06625 (LOY45_06620)	1434081..1434884	-	804	WP_134063779.1	aspartate dehydrogenase	-
LOY45_RS06630 (LOY45_06625)	1434895..1436577	-	1683	WP_115088233.1	thiamine pyrophosphate-binding protein	-
LOY45_RS06635 (LOY45_06630)	1436570..1437370	-	801	WP_258578881.1	SDR family oxidoreductase	-
LOY45_RS06640 (LOY45_06635)	1437372..1438199	-	828	WP_115088231.1	IclR family transcriptional regulator	Antitoxin
LOY45_RS06645 (LOY45_06640)	1438196..1439065	-	870	WP_233673369.1	alpha/beta fold hydrolase	Toxin
LOY45_RS06650 (LOY45_06645)	1439070..1439609	-	540	WP_115088229.1	cupin domain-containing protein	-
LOY45_RS06655 (LOY45_06650)	1439624..1440388	-	765	WP_115088510.1	SDR family oxidoreductase	-
LOY45_RS06660 (LOY45_06655)	1440388..1441164	-	777	WP_045193442.1	ABC transporter permease	-
LOY45_RS06665 (LOY45_06660)	1441194..1442192	-	999	WP_115088228.1	ABC transporter substrate-binding protein	-
LOY45_RS06670 (LOY45_06665)	1442232..1443068	-	837	WP_233673390.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (43-274)

Antitoxin

• Transcription regulator IclR, C-terminal (134-256)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30660.09 Da
• Isoelectric Point: 7.5101

>T225086 WP_233673369.1 NZ_CP087191:c1439065-1438196 [Pseudomonas wadenswilerensis]
MLHATDHALAALEAQLQQRFPQRLVPLAGGAQAIREAGHGPAIVLLHGIGSGAASWLQVALQLSPHARVIAWDAPGYGDS
SPLAAPAPQAADYARRLLQLLDALHIDDCVLVGHSLGALSAAALTHRQPQRVRRLVLISPARGYGDPARAEQARQVRSQR
LHNLEQLGISQMARQRSAHMLSPTASSDARAWVQWNMARLLPQGYRQAIELLCGDDLLRYAALAVPCDVYCGAADSITRP
EDCQALAKALGAAFALITEAGHASPIEQPEAVASLLARALEASLTGTAL

Nucleotide

• Length: 870 bp

>T225086 NZ_CP120949:c1018624-1018274 [Burkholderia contaminans]
ATGGTGAGGCGCGTCAAGTTCGAGAGGGGCGATATTGTGCGCGTTAGCCTGAACCCGACCGTGGGCAAGGAGCAACAAGG
GGATTTTCGCCCCGCGCTCGTTTTGTCGCCTGCTGCGTTCAATGCGCTTGGCGTGGCGCTCGTCGCGCCGATTACGCAGG
GCGGCGAATTCGCGCGATACGCGGGATTCGCTGTGCCCCTCTCGGGTTCGGGCACTGAAACGCAGGGCGTGGCTCTTGTG
AACATGGTTCGCACGCTCGATCTTCAGGCGCGTGGCGCACGTAAGGTCGAGCGCGCTCCGTCCGAAGTGATCGAAGATGC
GCTCGCGCGTCTTCAGACCATTCTCGAATAA

Antitoxin

Protein

• Length: 276 a.a.
• Molecular weight: 30455.85 Da
• Isoelectric Point: 6.4973

>AT225086 WP_115088231.1 NZ_CP087191:c1438199-1437372 [Pseudomonas wadenswilerensis]
MNDSDLLKDNQDKYIVPGLERGLLLLCEFSRQNRTLTAPELARRLELPRSTIFRLLTTLETMGFVTRSGNEYRLGMSVLR
LGFEYLASLELTELGQPLLARLCDHLNYPSNLVVRDGRSIVYVAKVSPPSVFSSAVNVGTRLPAHATVLGRILLEDLSLA
ELRELYPEEHLEQHSPCTPKTVLELFDLVQSDRQRGYVSGEGFFESSISTIAAPVRDHSGRVVAAMGVTIPTVQVGHINF
DGLLGHVRGSADELSRLLNYTPHAGSSRVTALMRD

Nucleotide

• Length: 828 bp

>AT225086 NZ_CP120949:c1018869-1018618 [Burkholderia contaminans]
ATGGAACTCAAAATTCAGAAGTGGGGCAACAGCGCCGCAGTCCGGCTTCCGGCCGTGTTGCTTGAGCAAATCGACGCGTC
GGTAGGTAGCTCGCTGAACGCCGATGTGCGCCCCGATGGTGTGTTGCTTTCGCCAGCCCGGCGCAAGTATTCACTGGATG
AACTGATTGCGCAGTGCGATCCGAAGGCCCCGGAACCCGCCGACCTTGCCGCGTGGGGCGAATCCAAGCCGGTGGGGCAT
GAAGCATGGTGA