Detailed information of TA system TA225080

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 2639022..2639611
• Replicon: chromosome
• Accession: NZ_CP087189
• Organism: Pseudomonas protegens strain B21-024

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7V8EXE5
• Locus tag: LOY29_RS12220
• Protein ID: WP_019094202.1
• Coordinates: 2639360..2639611 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LOY29_RS12215
• Protein ID: WP_072451388.1
• Coordinates: 2639022..2639363 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY29_RS12190 (LOY29_12175)	2634369..2635325	-	957	WP_015635066.1	nucleoside-specific channel-forming protein Tsx	-
LOY29_RS12195 (LOY29_12180)	2635588..2635989	+	402	WP_225368747.1	nuclear transport factor 2 family protein	-
LOY29_RS12200 (LOY29_12185)	2636033..2637067	+	1035	WP_015635068.1	selenide, water dikinase SelD	-
LOY29_RS12205 (LOY29_12190)	2637067..2638167	+	1101	WP_258611733.1	tRNA 2-selenouridine(34) synthase MnmH	-
LOY29_RS12210 (LOY29_12195)	2638220..2638561	-	342	WP_053157594.1	DUF3077 domain-containing protein	-
LOY29_RS12215 (LOY29_12200)	2639022..2639363	-	342	WP_072451388.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LOY29_RS12220 (LOY29_12205)	2639360..2639611	-	252	WP_019094202.1	type II toxin-antitoxin system HicA family toxin	Toxin
LOY29_RS12225 (LOY29_12210)	2639699..2640754	-	1056	WP_248766040.1	permease	-
LOY29_RS12230 (LOY29_12215)	2641343..2642119	-	777	WP_258611734.1	IclR family transcriptional regulator	-
LOY29_RS12235 (LOY29_12220)	2642291..2643061	+	771	WP_015635071.1	ABC transporter substrate-binding protein	-
LOY29_RS12240 (LOY29_12225)	2643122..2643778	+	657	WP_011060581.1	amino acid ABC transporter permease	-
LOY29_RS12245 (LOY29_12230)	2643775..2644521	+	747	WP_011060582.1	amino acid ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9239.70 Da
• Isoelectric Point: 10.4707

>T225080 WP_019094202.1 NZ_CP087189:c2639611-2639360 [Pseudomonas protegens]
VSKNDKLLAKLLNDRGTFSWQELVTLLKRLGYTPLEGSGSRVKFDNGDPGAMINLHKPHPGNELKAYIRRQVIEQLKSGG
LIP

Nucleotide

• Length: 252 bp

>T225080 NZ_CP087189:c2639611-2639360 [Pseudomonas protegens]
GTGTCCAAGAACGATAAGCTTCTCGCCAAGTTACTCAACGACCGAGGGACCTTTTCCTGGCAGGAACTGGTCACTCTCCT
AAAGCGCCTGGGCTACACACCGTTGGAGGGCTCGGGCAGCCGGGTAAAATTCGACAATGGTGATCCAGGCGCAATGATCA
ACCTGCACAAACCGCACCCCGGTAATGAGCTGAAGGCCTACATACGCCGCCAAGTGATCGAGCAACTGAAATCGGGAGGT
CTCATACCGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12438.82 Da
• Isoelectric Point: 4.8281

>AT225080 WP_072451388.1 NZ_CP087189:c2639363-2639022 [Pseudomonas protegens]
VNNLLQHKGYYGSIEVSTEDNCLFGKLQFIRALVSYEGQTVAELEAAFKEAVDDYLDTCEQSGTAPEIPCKGSFNVRVGH
DLHLAAAVAATRQNVSLNDLTRQALNEYLQHHG

Nucleotide

• Length: 342 bp

>AT225080 NZ_CP087189:c2639363-2639022 [Pseudomonas protegens]
GTGAACAACCTACTGCAGCACAAGGGCTATTACGGCTCTATCGAGGTCAGTACCGAGGACAATTGCCTCTTTGGCAAACT
TCAGTTCATAAGGGCCTTGGTTAGCTATGAGGGGCAGACCGTGGCTGAACTCGAAGCGGCCTTCAAAGAGGCAGTAGACG
ACTATCTGGATACCTGTGAGCAATCGGGTACAGCACCCGAAATCCCTTGCAAAGGCTCGTTCAACGTTCGGGTAGGCCAC
GATCTTCATCTAGCGGCTGCCGTGGCCGCCACCCGGCAGAATGTATCGTTGAACGATCTGACTCGTCAGGCCCTGAATGA
ATACCTGCAACATCACGGCTGA

Structures

Toxin

• AlphaFold DB: A0A7V8EXE5