Detailed information of TA system TA022508

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4821527..4822119
• Replicon: chromosome
• Accession: NC_009937
• Organism: Azorhizobium caulinodans ORS 571

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A8HSX8
• Locus tag: AZC_RS21715
• Protein ID: WP_012172750.1
• Coordinates: 4821527..4821781 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AZC_RS21720
• Protein ID: WP_043879718.1
• Coordinates: 4821778..4822119 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZC_RS21695	4817135..4818616	-	1482	WP_012172746.1	ammonium transporter	-
AZC_RS21700	4818647..4818985	-	339	WP_012172747.1	P-II family nitrogen regulator	-
AZC_RS21705	4819248..4820111	-	864	WP_043879717.1	acyl-CoA thioesterase II	-
AZC_RS21710	4820243..4821478	+	1236	WP_012172749.1	ubiquinone biosynthesis hydroxylase	-
AZC_RS21715	4821527..4821781	+	255	WP_012172750.1	type II toxin-antitoxin system HicA family toxin	Toxin
AZC_RS21720	4821778..4822119	+	342	WP_043879718.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AZC_RS21725	4822159..4822371	-	213	WP_043879719.1	Trm112 family protein	-
AZC_RS21730	4822573..4823472	+	900	WP_012172753.1	DMT family transporter	-
AZC_RS21735	4823515..4825023	-	1509	WP_012172754.1	histidine--tRNA ligase	-
AZC_RS21740	4825080..4825895	-	816	WP_043879720.1	MOSC domain-containing protein	-
AZC_RS21745	4826024..4826482	+	459	WP_043880611.1	PaaI family thioesterase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-75)

Antitoxin

• Uncharacterised protein family HicB (56-105)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9306.66 Da
• Isoelectric Point: 10.7408

>T22508 WP_012172750.1 NC_009937:4821527-4821781 [Azorhizobium caulinodans ORS 571]
VKKRHLATLELIFSHPVSGSVRWSDVVALFQALGAEVSEREGSRVGVVLFGEVRVFHRPHPSPNTDKGAVASIRKWLDEH
GVRP

Nucleotide

• Length: 255 bp

>T22508 NC_009937:4821527-4821781 [Azorhizobium caulinodans ORS 571]
GTGAAGAAGCGCCACCTGGCCACGCTTGAGCTGATCTTTTCGCATCCGGTGAGCGGCTCCGTCCGCTGGTCGGATGTCGT
GGCTCTGTTTCAAGCGCTGGGCGCCGAGGTGAGCGAGCGGGAAGGGTCGCGTGTGGGCGTGGTGCTGTTCGGCGAAGTGC
GGGTGTTTCACCGTCCACACCCTTCGCCCAACACGGACAAGGGCGCCGTCGCCAGCATTCGCAAGTGGCTCGACGAACAC
GGAGTTCGGCCATGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12151.65 Da
• Isoelectric Point: 4.3324

>AT22508 WP_043879718.1 NC_009937:4821778-4822119 [Azorhizobium caulinodans ORS 571]
MMNIIEIDGEKAVVAFDPDIRMFRGEFVGLNGGADFYAETVEQLYEEGRTSLAAFLALCAEKGIAPRRTFSGRFNVRLDP
ADHEAAVVAASAQGKSLNEWVAAAIRDAADNAA

Nucleotide

• Length: 342 bp

>AT22508 NC_009937:4821778-4822119 [Azorhizobium caulinodans ORS 571]
ATGATGAACATCATCGAGATCGACGGCGAAAAGGCCGTGGTGGCCTTCGATCCGGACATCCGGATGTTCCGGGGGGAGTT
CGTCGGGTTGAACGGTGGGGCCGATTTCTACGCCGAGACCGTTGAGCAGTTGTACGAGGAGGGGCGCACGTCGCTCGCCG
CTTTCCTCGCGCTCTGTGCCGAGAAGGGGATCGCGCCACGGCGTACCTTCTCGGGCCGCTTCAACGTGCGGCTGGACCCT
GCCGACCATGAGGCCGCCGTGGTGGCCGCCTCCGCTCAGGGAAAGAGCCTGAATGAGTGGGTTGCCGCCGCGATTCGTGA
CGCGGCGGACAATGCAGCCTGA

Structures

Toxin

• AlphaFold DB: A8HSX8