Detailed information of TA system TA225079

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2534858..2535477
• Replicon: chromosome
• Accession: NZ_CP087189
• Organism: Pseudomonas protegens strain B21-024

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2J7U9Z9
• Locus tag: LOY29_RS11705
• Protein ID: WP_041120604.1
• Coordinates: 2535295..2535477 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LOY29_RS11700
• Protein ID: WP_258611705.1
• Coordinates: 2534858..2535259 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY29_RS11670 (LOY29_11660)	2530309..2530656	-	348	WP_011060486.1	metalloregulator ArsR/SmtB family transcription factor	-
LOY29_RS11675 (LOY29_11665)	2530887..2531441	+	555	WP_186452724.1	hypothetical protein	-
LOY29_RS11680 (LOY29_11670)	2531686..2532117	-	432	WP_011060488.1	carboxymuconolactone decarboxylase family protein	-
LOY29_RS11685 (LOY29_11675)	2532146..2532766	-	621	WP_103408227.1	NAD(P)H-dependent oxidoreductase	-
LOY29_RS11690 (LOY29_11680)	2532763..2533209	-	447	WP_258611703.1	GNAT family N-acetyltransferase	-
LOY29_RS11695 (LOY29_11685)	2533297..2534700	+	1404	WP_258611704.1	PLP-dependent aminotransferase family protein	-
LOY29_RS11700 (LOY29_11690)	2534858..2535259	-	402	WP_258611705.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LOY29_RS11705 (LOY29_11695)	2535295..2535477	-	183	WP_041120604.1	type II toxin-antitoxin system HicA family toxin	Toxin
LOY29_RS11710 (LOY29_11700)	2535878..2536138	-	261	WP_258611706.1	DUF6429 family protein	-
LOY29_RS11715 (LOY29_11705)	2536271..2536846	-	576	WP_103291340.1	hypothetical protein	-
LOY29_RS11720 (LOY29_11710)	2537382..2537900	+	519	WP_080966932.1	sugar O-acetyltransferase	-
LOY29_RS11725 (LOY29_11715)	2537921..2539159	+	1239	WP_019094712.1	aminotransferase class III-fold pyridoxal phosphate-dependent enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6885.09 Da
• Isoelectric Point: 11.2684

>T225079 WP_041120604.1 NZ_CP087189:c2535477-2535295 [Pseudomonas protegens]
VQSRQLIKELEAAGWVLERVTGSHHLFKHPYRPNTVPVPHPKKDLPRGTVRAIRKLAELI

Nucleotide

• Length: 183 bp

>T225079 NZ_CP120947:1933372-1933611 [Burkholderia contaminans]
ATTTGGCGCGATACCGTCGGGCGTGGGTCGCTCAAGCAAACCGACCTTTACCACCGCGATCTGATCGGAACCATGGAAGC
GCTGGCGCGCGGTGAAAAGGTCGGCCGGACTTGCATTGTTCGCGATGGATATTTCCGGTACGCCGTGGGCTCCCACGTCG
TGTTCTATCGTGAAACGGAATACACGATCGATTTGATTCGGGTCCTGCATCAACGCATGGACATCGAACAACATCTGTAG

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14563.50 Da
• Isoelectric Point: 4.7182

>AT225079 WP_258611705.1 NZ_CP087189:c2535259-2534858 [Pseudomonas protegens]
VQYPICIEWGDEHTATGIQIPDIPGAVTAGDSFESAYSAAIEIAHVMLEELARAGNAIPLPSPTRNHRANPDFEGMGWGM
LDIDIAPYLGKTEKVNVTLPGYVIQQIDRFVREHNIKSRSSFLADAAMEKLGR

Nucleotide

• Length: 402 bp

>AT225079 NZ_CP120947:1933081-1933326 [Burkholderia contaminans]
ATGTCGCAAAACGCATCCGTTTCCCGGCGCGACCACTTTGCCGAACTCGTCGATACCCAAGTGCAATCGGAACAATACGG
TTCCGCAAGCGACGTCGAACGAACCGGTTTGCGGCTTCTCGAATCACGCGACATGCAGGTGCGCGCGTTGCAGGAAGCGT
TGAAGGCCGGGGAGGTATCGGGAGCACTCAGGCCATTCGACCGCGAGGCCTTCCTGGCCCGCATGCGGGCCGCGCATGAC
GGCTAA

Structures

Toxin

• AlphaFold DB: A0A2J7U9Z9