Detailed information of TA system TA225075

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 6485777..6487404
• Replicon: chromosome
• Accession: NZ_CP087188
• Organism: Pseudomonas laurylsulfatiphila strain B21-025

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LOY25_RS29340
• Protein ID: WP_258682959.1
• Coordinates: 6486052..6487404 (-)
• Length: 451 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LOY25_RS29335
• Protein ID: WP_007971709.1
• Coordinates: 6485777..6486055 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY25_RS29315 (LOY25_29270)	6482218..6483771	-	1554	WP_258682957.1	PLP-dependent aminotransferase family protein	-
LOY25_RS29320 (LOY25_29275)	6483864..6484487	+	624	WP_007971713.1	FMN-binding negative transcriptional regulator	-
LOY25_RS29325 (LOY25_29280)	6484498..6484944	+	447	WP_116579791.1	GNAT family N-acetyltransferase	-
LOY25_RS29330 (LOY25_29285)	6484962..6485633	+	672	WP_258682958.1	GNAT family protein	-
LOY25_RS29335 (LOY25_29290)	6485777..6486055	-	279	WP_007971709.1	helix-turn-helix transcriptional regulator	Antitoxin
LOY25_RS29340 (LOY25_29295)	6486052..6487404	-	1353	WP_258682959.1	HipA domain-containing protein	Toxin
LOY25_RS29345 (LOY25_29300)	6487731..6489554	-	1824	WP_007971704.1	sodium-extruding oxaloacetate decarboxylase subunit alpha	-
LOY25_RS29350 (LOY25_29305)	6489566..6490981	-	1416	WP_007971702.1	acetyl-CoA carboxylase biotin carboxylase subunit	-
LOY25_RS29355 (LOY25_29310)	6491174..6492136	+	963	WP_218473044.1	LysR family transcriptional regulator	-
LOY25_RS29360 (LOY25_29315)	6492078..6492287	-	210	WP_007971698.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	6485777..6494039	8262

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (165-367)

Antitoxin

• Cro/C1-type helix-turn-helix domain (27-80)

Sequences

Toxin

Protein

• Length: 451 a.a.
• Molecular weight: 50143.55 Da
• Isoelectric Point: 4.7833

>T225075 WP_258682959.1 NZ_CP087188:c6487404-6486052 [Pseudomonas laurylsulfatiphila]
MYDLTLQIYWAGNWHDAMILSFDSPEKGFESRCNFGYQQTYLFENYEDIGTPFAKAVSARFPLDWDGRRSNAPAFVHDVA
PAGAAKRFLLAHIGKDKPADVSADLYLLARSTPAPIGNMRVKASAEALDQREPIGFERQDVISRNNRFLEYAYEQGAAIG
GATGAGGEAPKLLLAQNKAGLLYPDAVLGDADVTQHWFVKFARNKGLETDQVILRSEYHYYRALQSLGIETVAVEGLALE
EATKPSLWMQRFDRQVTDQGVERFAVESIYSLANITTPGSALDHMDVIRLLAGLWREAGQADQIPDLVADYLRRDLINKI
LGNSDNHGRNTAIIRDDASFRLAPIYDLAPMVMDDEGVTRTTKWPKELERAGDVDWRGVCRTLADIADPDDPLAALSDVQ
DSFERLREDATRLMALPDILSASGLPGQTMNHPQIALKNLEQRLKEWGLK

Nucleotide

• Length: 1353 bp

>T225075 NZ_CP120945:c89495-89190 [Escherichia coli O157:H7 str. EDL933]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10302.09 Da
• Isoelectric Point: 10.7364

>AT225075 WP_007971709.1 NZ_CP087188:c6486055-6485777 [Pseudomonas laurylsulfatiphila]
MSMTVAERTVLIESIQEALAQGTLEIGEAVRRLRVEVTGLHQTQFAKMCKISVRTLVHIEHGEGNQTLKSLNAVFRPFGL
KMGVVRIRRDIS

Nucleotide

• Length: 279 bp

>AT225075 NZ_CP120945:c89715-89497 [Escherichia coli O157:H7 str. EDL933]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA