Detailed information of TA system TA225073

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 2265834..2266491
• Replicon: chromosome
• Accession: NZ_CP087188
• Organism: Pseudomonas laurylsulfatiphila strain B21-025

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LOY25_RS10255
• Protein ID: WP_258685369.1
• Coordinates: 2266309..2266491 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LOY25_RS10250
• Protein ID: WP_258685367.1
• Coordinates: 2265834..2266259 (-)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY25_RS10200 (LOY25_10175)	2261131..2261793	+	663	WP_258685347.1	replication protein P	-
LOY25_RS10205 (LOY25_10180)	2261786..2261995	+	210	WP_258685349.1	hypothetical protein	-
LOY25_RS10210 (LOY25_10185)	2261986..2262288	+	303	WP_258685351.1	DUF1364 domain-containing protein	-
LOY25_RS10215 (LOY25_10190)	2262285..2262659	+	375	WP_258685353.1	endodeoxyribonuclease RusA	-
LOY25_RS10220 (LOY25_10195)	2262656..2263936	+	1281	WP_258685355.1	site-specific integrase	-
LOY25_RS10225 (LOY25_10200)	2263939..2264139	+	201	WP_258685357.1	hypothetical protein	-
LOY25_RS10230 (LOY25_10205)	2264136..2264456	+	321	WP_258685360.1	hypothetical protein	-
LOY25_RS10235 (LOY25_10210)	2264466..2265029	+	564	WP_258685361.1	hypothetical protein	-
LOY25_RS10240 (LOY25_10215)	2265195..2265395	+	201	WP_258685363.1	hypothetical protein	-
LOY25_RS10245 (LOY25_10220)	2265392..2265724	+	333	WP_258685365.1	hypothetical protein	-
LOY25_RS10250 (LOY25_10225)	2265834..2266259	-	426	WP_258685367.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LOY25_RS10255 (LOY25_10230)	2266309..2266491	-	183	WP_258685369.1	type II toxin-antitoxin system HicA family toxin	Toxin
LOY25_RS10260 (LOY25_10235)	2266688..2267017	+	330	WP_258685371.1	phage holin, lambda family	-
LOY25_RS10265 (LOY25_10240)	2267138..2267476	+	339	WP_258685372.1	HNH endonuclease signature motif containing protein	-
LOY25_RS10270 (LOY25_10245)	2267576..2268022	+	447	WP_258685374.1	terminase small subunit	-
LOY25_RS10275 (LOY25_10250)	2268023..2269699	+	1677	WP_258685376.1	terminase large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2247897..2298926	51029

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-63)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6977.13 Da
• Isoelectric Point: 10.6301

>T225073 WP_258685369.1 NZ_CP087188:c2266491-2266309 [Pseudomonas laurylsulfatiphila]
MKYSEFRRWLKARGVIFEAGKGTSHFKIYYNGKQTVFADHGAKEMKEPTRKGIIKQLGLE

Nucleotide

• Length: 183 bp

>T225073 NZ_CP120944:c4955890-4955717 [Escherichia coli O157:H7 str. EDL933]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15403.67 Da
• Isoelectric Point: 4.5365

>AT225073 WP_258685367.1 NZ_CP087188:c2266259-2265834 [Pseudomonas laurylsulfatiphila]
MYDYPVIIHEDENPGVAVTAPDLPEFNSAGDDVADALRESVDGIETALSIYVDQRRVIPPASQPEPGQHVVRLPAVTVAK
IVLWNEMMARDMRKADLCRLLGLAQTQGDRLVDFLHTSKMEAIEKALAALGKRLVVSVEAA

Nucleotide

• Length: 426 bp

>AT225073 NZ_CP120944:c4956214-4955918 [Escherichia coli O157:H7 str. EDL933]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG