Detailed information of TA system TA225072

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1820267..1821306
• Replicon: chromosome
• Accession: NZ_CP087188
• Organism: Pseudomonas laurylsulfatiphila strain B21-025

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: LOY25_RS08130
• Protein ID: WP_126046731.1
• Coordinates: 1820267..1820842 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: LOY25_RS08135
• Protein ID: WP_218475322.1
• Coordinates: 1820839..1821306 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY25_RS08105 (LOY25_08085)	1816234..1816770	+	537	WP_218481597.1	DsbE family thiol:disulfide interchange protein	-
LOY25_RS08110 (LOY25_08090)	1816767..1817240	+	474	WP_104449888.1	cytochrome c-type biogenesis protein CcmH	-
LOY25_RS08115 (LOY25_08095)	1817233..1818435	+	1203	WP_258684902.1	c-type cytochrome biogenesis protein CcmI	-
LOY25_RS08120 (LOY25_08100)	1818451..1818858	+	408	WP_007980770.1	hypothetical protein	-
LOY25_RS08125 (LOY25_08105)	1819603..1820169	+	567	WP_258684905.1	phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN	-
LOY25_RS08130 (LOY25_08110)	1820267..1820842	-	576	WP_126046731.1	PIN domain-containing protein	Toxin
LOY25_RS08135 (LOY25_08115)	1820839..1821306	-	468	WP_218475322.1	helix-turn-helix domain-containing protein	Antitoxin
LOY25_RS08140 (LOY25_08120)	1821390..1822277	-	888	WP_258684908.1	LysR family transcriptional regulator	-
LOY25_RS08145 (LOY25_08125)	1822324..1823529	-	1206	WP_258684909.1	MFS transporter	-
LOY25_RS08150 (LOY25_08130)	1823775..1824770	-	996	WP_258684911.1	sulfate ABC transporter substrate-binding protein	-
LOY25_RS08155 (LOY25_08135)	1824864..1825688	-	825	WP_007971419.1	ion transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21437.69 Da
• Isoelectric Point: 6.8454

>T225072 WP_126046731.1 NZ_CP087188:c1820842-1820267 [Pseudomonas laurylsulfatiphila]
VRHSSFTAVYDACVLYPAPLRDFLMWLALSGLFRAKWSVAIHDEWKRNLLKNRPDLNATQLNRTCELMDLAIPDACVSGY
EALIQGLTLPDADDRHVLAAAIRCNASVIVTFNQKDFPRTALQPYGIEAQHPDEFVNDLFDLDPAAVVAAAQRQRKQLKA
PPMNTDTYLDLLLRQGLVQSVKALNSFRAML

Nucleotide

• Length: 576 bp

>T225072 NZ_CP120944:c4859076-4858726 [Escherichia coli O157:H7 str. EDL933]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17323.74 Da
• Isoelectric Point: 6.2912

>AT225072 WP_218475322.1 NZ_CP087188:c1821306-1820839 [Pseudomonas laurylsulfatiphila]
MSTVIHPPINLPAKHEIQAAIEGQRTLAAYLATQFETQHIQIFDEQNEAHNIELPTSALRLLVDILAELAAGNAVKVVPV
HAELTTQEAADLLNVSRPHLIKLLESGELSYHKTGKHRRVRFADLMDYKNRRDTASEQAMLLLAEQAQALRTGYE

Nucleotide

• Length: 468 bp

>AT225072 NZ_CP120944:c4859321-4859070 [Escherichia coli O157:H7 str. EDL933]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA