Detailed information of TA system TA225069

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 174790..175306
• Replicon: chromosome
• Accession: NZ_CP087188
• Organism: Pseudomonas laurylsulfatiphila strain B21-025

Toxin (Protein)

• Gene name: relE
• Uniprot ID: J2YKB6
• Locus tag: LOY25_RS00745
• Protein ID: WP_007975642.1
• Coordinates: 175025..175306 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: J2QY16
• Locus tag: LOY25_RS00740
• Protein ID: WP_007975643.1
• Coordinates: 174790..175035 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY25_RS00710 (LOY25_00710)	170247..171761	+	1515	WP_116582502.1	TolC family protein	-
LOY25_RS00715 (LOY25_00715)	171745..172350	+	606	WP_258683061.1	adenylyl-sulfate kinase	-
LOY25_RS00720 (LOY25_00720)	172362..173120	-	759	WP_218475545.1	slipin family protein	-
LOY25_RS00725 (LOY25_00725)	173123..173662	-	540	WP_258683062.1	NfeD family protein	-
LOY25_RS00730 (LOY25_00730)	173862..174323	+	462	WP_217836835.1	YbaK/EbsC family protein	-
LOY25_RS00735 (LOY25_00735)	174462..174707	+	246	WP_007975644.1	DUF2789 domain-containing protein	-
LOY25_RS00740 (LOY25_00740)	174790..175035	+	246	WP_007975643.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LOY25_RS00745 (LOY25_00745)	175025..175306	+	282	WP_007975642.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOY25_RS00750 (LOY25_00750)	175439..175867	+	429	WP_033047983.1	winged helix DNA-binding protein	-
LOY25_RS00755 (LOY25_00755)	175864..177936	+	2073	WP_258683063.1	FUSC family protein	-
LOY25_RS00760 (LOY25_00760)	177933..178142	+	210	WP_007975639.1	DUF1656 domain-containing protein	-
LOY25_RS00765 (LOY25_00765)	178139..179026	+	888	WP_258683064.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-71)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11082.90 Da
• Isoelectric Point: 10.3652

>T225069 WP_007975642.1 NZ_CP087188:175025-175306 [Pseudomonas laurylsulfatiphila]
MTYKLEFLPSARKEWDKLGHTVREQFKKKLSERLEVPRIQSDALHGMPDCYKIKLRSSGYRLVYEVIDERIVVSVVAVGK
RERSEVYERARKR

Nucleotide

• Length: 282 bp

>T225069 NZ_CP120944:c4374601-4374203 [Escherichia coli O157:H7 str. EDL933]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8896.26 Da
• Isoelectric Point: 4.7124

>AT225069 WP_007975643.1 NZ_CP087188:174790-175035 [Pseudomonas laurylsulfatiphila]
MQNVLADMAVSVSELKKNPSAVLNGAHGGPVAVLNHNRVMGYMVPADVFEAMMERLDDLELAELVRSRRHETPIPVTLDD
L

Nucleotide

• Length: 246 bp

>AT225069 NZ_CP120944:c4374897-4374604 [Escherichia coli O157:H7 str. EDL933]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: J2YKB6

Antitoxin

• AlphaFold DB: J2QY16