Detailed information of TA system TA225068

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-YefM
• Location: 4825878..4826383
• Replicon: chromosome
• Accession: NZ_CP087187
• Organism: Pseudomonas atacamensis strain B21-026

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LOY41_RS21605
• Protein ID: WP_073473381.1
• Coordinates: 4826129..4826383 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A1N7ATY6
• Locus tag: LOY41_RS21600
• Protein ID: WP_042608352.1
• Coordinates: 4825878..4826132 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY41_RS21570 (LOY41_21535)	4821121..4821339	-	219	WP_241089700.1	DUF6429 family protein	-
LOY41_RS21575 (LOY41_21540)	4821560..4821889	+	330	WP_122706599.1	hypothetical protein	-
LOY41_RS21580 (LOY41_21545)	4821908..4822276	-	369	WP_039761415.1	PH domain-containing protein	-
LOY41_RS21585 (LOY41_21550)	4822352..4822810	+	459	WP_146226152.1	hypothetical protein	-
LOY41_RS21590 (LOY41_21555)	4822903..4823655	+	753	WP_122706603.1	hypothetical protein	-
LOY41_RS21595 (LOY41_21560)	4823727..4825646	+	1920	WP_258717368.1	alkaline phosphatase D family protein	-
LOY41_RS21600 (LOY41_21565)	4825878..4826132	+	255	WP_042608352.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LOY41_RS21605 (LOY41_21570)	4826129..4826383	+	255	WP_073473381.1	Txe/YoeB family addiction module toxin	Toxin
LOY41_RS21610 (LOY41_21575)	4826377..4827564	-	1188	WP_122706607.1	aspartate aminotransferase family protein	-
LOY41_RS21615 (LOY41_21580)	4827667..4828587	+	921	WP_110646188.1	LysR family transcriptional regulator	-
LOY41_RS21620 (LOY41_21585)	4828626..4829030	+	405	WP_073473390.1	GNAT family N-acetyltransferase	-
LOY41_RS21625 (LOY41_21590)	4829164..4829865	+	702	WP_122706609.1	hypothetical protein	-
LOY41_RS21630 (LOY41_21595)	4829934..4830659	-	726	WP_122706611.1	phosphocholine cytidylyltransferase family protein	-
LOY41_RS21635 (LOY41_21600)	4830656..4831258	-	603	WP_073473398.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9981.56 Da
• Isoelectric Point: 9.0627

>T225068 WP_073473381.1 NZ_CP087187:4826129-4826383 [Pseudomonas atacamensis]
MKIEFTPTGWEDYLWFQQNDKAGLKRINLLIKAIKREPFEGVGKPEALKHNMSGLWSRRITAEHRLVYRVEDGEICVISC
RYHY

Nucleotide

• Length: 255 bp

>T225068 NZ_CP120944:4118416-4118634 [Escherichia coli O157:H7 str. EDL933]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9503.96 Da
• Isoelectric Point: 7.0130

>AT225068 WP_042608352.1 NZ_CP087187:4825878-4826132 [Pseudomonas atacamensis]
MQTINYTTARAHLAETMDRVNEDRAPLLVTRQKGEPVVMMSLAEYNALEETAYLLRSPANAERLMKSIGELRAGKAKVRQ
LIEE

Nucleotide

• Length: 255 bp

>AT225068 NZ_CP120944:4118016-4118390 [Escherichia coli O157:H7 str. EDL933]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A1N7ATY6