Detailed information of TA system TA225063

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1438701..1439323
• Replicon: chromosome
• Accession: NZ_CP087187
• Organism: Pseudomonas atacamensis strain B21-026

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A4S3YXS2
• Locus tag: LOY41_RS06285
• Protein ID: WP_016771297.1
• Coordinates: 1439144..1439323 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V8RAB1
• Locus tag: LOY41_RS06280
• Protein ID: WP_024011974.1
• Coordinates: 1438701..1439108 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY41_RS06255 (LOY41_06245)	1434378..1435727	+	1350	WP_258717446.1	sensor domain-containing diguanylate cyclase	-
LOY41_RS06260 (LOY41_06250)	1435881..1436384	+	504	WP_110644971.1	DUF4142 domain-containing protein	-
LOY41_RS06265 (LOY41_06255)	1436546..1436956	-	411	WP_016771301.1	low affinity iron permease family protein	-
LOY41_RS06270 (LOY41_06260)	1437116..1437886	+	771	WP_110644972.1	trans-aconitate 2-methyltransferase	-
LOY41_RS06275 (LOY41_06265)	1438414..1438641	-	228	Protein_1232	transcriptional regulator	-
LOY41_RS06280 (LOY41_06270)	1438701..1439108	-	408	WP_024011974.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LOY41_RS06285 (LOY41_06275)	1439144..1439323	-	180	WP_016771297.1	type II toxin-antitoxin system HicA family toxin	Toxin
LOY41_RS06290 (LOY41_06280)	1439396..1439656	-	261	WP_163032966.1	hypothetical protein	-
LOY41_RS06295 (LOY41_06285)	1439787..1441211	-	1425	WP_163032960.1	IS1182 family transposase	-
LOY41_RS06305 (LOY41_06295)	1441589..1442473	-	885	WP_016771295.1	alpha/beta hydrolase	-
LOY41_RS06310 (LOY41_06300)	1442790..1443482	-	693	WP_122704859.1	pseudouridine synthase	-
LOY41_RS06315 (LOY41_06305)	1443516..1443746	-	231	WP_073473113.1	cysteine-rich CWC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1439787..1441211	1424

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6676.97 Da
• Isoelectric Point: 11.8222

>T225063 WP_016771297.1 NZ_CP087187:c1439323-1439144 [Pseudomonas atacamensis]
VNSRFLIGQLVADGWFLVRIRGSHHHFKHPTKPGLVTVPHPKKDLLKKTAISILQQAML

Nucleotide

• Length: 180 bp

>T225063 NZ_CP120944:2676886-2677062 [Escherichia coli O157:H7 str. EDL933]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14485.45 Da
• Isoelectric Point: 4.4796

>AT225063 WP_024011974.1 NZ_CP087187:c1439108-1438701 [Pseudomonas atacamensis]
MLYPIAISIGDEEHAWGVEVPDIPGCFSAGDDLDDAVAMAREAIEGHFEILADDGAPIPSASKLSLHVANPQYAGCAWAV
VDIDVVKYLGKAQKLNITLPGHLLNRIDEYVLHHPEEKSRSGFLASAALKVLQQG

Nucleotide

• Length: 408 bp

>AT225063 NZ_CP120944:2677108-2677524 [Escherichia coli O157:H7 str. EDL933]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCAGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A4S3YXS2

Antitoxin

• AlphaFold DB: V8RAB1