Detailed information of TA system TA225061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 326055..326683
• Replicon: chromosome
• Accession: NZ_CP087187
• Organism: Pseudomonas atacamensis strain B21-026

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LOY41_RS01425
• Protein ID: WP_016772140.1
• Coordinates: 326055..326453 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V8RDT4
• Locus tag: LOY41_RS01430
• Protein ID: WP_024011210.1
• Coordinates: 326453..326683 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY41_RS01405 (LOY41_01405)	321729..322424	-	696	WP_122705208.1	ABC transporter permease	-
LOY41_RS01410 (LOY41_01410)	322502..323254	-	753	WP_122705206.1	ABC transporter substrate-binding protein	-
LOY41_RS01415 (LOY41_01415)	323268..324041	-	774	WP_016772142.1	ABC transporter ATP-binding protein	-
LOY41_RS01420 (LOY41_01420)	324585..325976	+	1392	WP_016772141.1	GABA permease	-
LOY41_RS01425 (LOY41_01425)	326055..326453	-	399	WP_016772140.1	tRNA(fMet)-specific endonuclease VapC	Toxin
LOY41_RS01430 (LOY41_01430)	326453..326683	-	231	WP_024011210.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LOY41_RS01435 (LOY41_01435)	326849..327301	-	453	WP_122705204.1	hypothetical protein	-
LOY41_RS01440 (LOY41_01440)	327308..327721	-	414	WP_016772137.1	hypothetical protein	-
LOY41_RS01445 (LOY41_01445)	327751..328686	-	936	Protein_280	alpha/beta fold hydrolase	-
LOY41_RS01450 (LOY41_01450)	328793..329851	-	1059	WP_122705202.1	PDDEXK nuclease domain-containing protein	-
LOY41_RS01455 (LOY41_01455)	330133..330477	-	345	WP_122705200.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14547.92 Da
• Isoelectric Point: 7.1340

>T225061 WP_016772140.1 NZ_CP087187:c326453-326055 [Pseudomonas atacamensis]
MIKYMLDTNICIFTIKNKPQIVREAFNRHSGQLCISAITLMELIYGAEKSAAPEKNLAIVEGFVARLDVLPFDNDAAAQA
GMVRSELAKAGTPIGPYDQMIAGHARSLGLIVVTNNVREFQRVSGLRIEDWV

Nucleotide

• Length: 399 bp

>T225061 NZ_CP120944:c2414023-2413745 [Escherichia coli O157:H7 str. EDL933]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8588.62 Da
• Isoelectric Point: 4.5203

>AT225061 WP_024011210.1 NZ_CP087187:c326683-326453 [Pseudomonas atacamensis]
MEQTTIFMSNRSQAVRLPKAVAMPGDVKRVEVIAIGRTRVITPAGESWDSWFDGDSVSPDFMANREQPTEQERESF

Nucleotide

• Length: 231 bp

>AT225061 NZ_CP120944:c2414216-2414025 [Escherichia coli O157:H7 str. EDL933]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Antitoxin

• AlphaFold DB: V8RDT4