Detailed information of TA system TA225059

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 174135..174651
• Replicon: chromosome
• Accession: NZ_CP087187
• Organism: Pseudomonas atacamensis strain B21-026

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LOY41_RS00735
• Protein ID: WP_071174010.1
• Coordinates: 174370..174651 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2U1R4X8
• Locus tag: LOY41_RS00730
• Protein ID: WP_041477418.1
• Coordinates: 174135..174380 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY41_RS00710 (LOY41_00710)	169523..171061	+	1539	WP_122705342.1	MFS transporter	-
LOY41_RS00715 (LOY41_00715)	171089..172156	+	1068	WP_122705340.1	HlyD family secretion protein	-
LOY41_RS00720 (LOY41_00720)	172153..173721	+	1569	WP_206074999.1	efflux transporter outer membrane subunit	-
LOY41_RS00725 (LOY41_00725)	173800..174045	+	246	WP_122705336.1	DUF2789 domain-containing protein	-
LOY41_RS00730 (LOY41_00730)	174135..174380	+	246	WP_041477418.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LOY41_RS00735 (LOY41_00735)	174370..174651	+	282	WP_071174010.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOY41_RS00740 (LOY41_00740)	175013..175441	+	429	WP_016772266.1	winged helix DNA-binding protein	-
LOY41_RS00745 (LOY41_00745)	175438..177501	+	2064	WP_122705334.1	FUSC family protein	-
LOY41_RS00750 (LOY41_00750)	177498..177707	+	210	WP_016772264.1	DUF1656 domain-containing protein	-
LOY41_RS00755 (LOY41_00755)	177704..178591	+	888	WP_071174012.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10842.73 Da
• Isoelectric Point: 10.5812

>T225059 WP_071174010.1 NZ_CP087187:174370-174651 [Pseudomonas atacamensis]
MTYKLEFLPSAHKEWNKLGHTLREQFKKKLGERLKLPRIPADALHSMPDCYKIKLKASGYRLVYQVIDERVVVSVLAVGK
RERSSVYDAARNR

Nucleotide

• Length: 282 bp

>T225059 NZ_CP120944:1036473-1036808 [Escherichia coli O157:H7 str. EDL933]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8806.13 Da
• Isoelectric Point: 4.4988

>AT225059 WP_041477418.1 NZ_CP087187:174135-174380 [Pseudomonas atacamensis]
MQSVLADMAVSVSELKKNPTAVLNGAHGGAVAVLNHNRVMGYMVPAEVYEAMMERLDDLELADIVRSRLHETPISVSLDD
L

Nucleotide

• Length: 246 bp

>AT225059 NZ_CP120944:1036225-1036473 [Escherichia coli O157:H7 str. EDL933]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2U1R4X8