Detailed information of TA system TA225054

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 2286687..2287167
• Replicon: chromosome
• Accession: NZ_CP087186
• Organism: Pseudomonas siliginis strain B21-063

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LOY48_RS09955
• Protein ID: WP_252879826.1
• Coordinates: 2286687..2286971 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LOY48_RS09960
• Protein ID: WP_123463680.1
• Coordinates: 2286961..2287167 (-)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY48_RS09920 (LOY48_09900)	2282547..2283020	+	474	WP_252885722.1	type II secretion system protein	-
LOY48_RS09925 (LOY48_09905)	2283026..2283400	+	375	WP_122610261.1	type II secretion system protein	-
LOY48_RS09930 (LOY48_09910)	2283375..2283914	+	540	WP_150741547.1	type II secretion system protein	-
LOY48_RS09935 (LOY48_09915)	2283911..2284303	+	393	WP_038364891.1	curli production assembly/transport protein CsgE	-
LOY48_RS09940 (LOY48_09920)	2284300..2284731	+	432	WP_258707909.1	curli assembly protein CsgF	-
LOY48_RS09945 (LOY48_09925)	2284761..2285621	+	861	WP_150741549.1	CsgG/HfaB family protein	-
LOY48_RS09950 (LOY48_09930)	2286142..2286498	+	357	WP_258707910.1	DUF6124 family protein	-
LOY48_RS09955 (LOY48_09935)	2286687..2286971	-	285	WP_252879826.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOY48_RS09960 (LOY48_09940)	2286961..2287167	-	207	WP_123463680.1	antitoxin	Antitoxin
LOY48_RS09965 (LOY48_09945)	2287444..2288895	-	1452	WP_258707911.1	curlin	-
LOY48_RS09970 (LOY48_09950)	2288923..2289387	-	465	WP_150741552.1	curlin	-
LOY48_RS09975 (LOY48_09955)	2289459..2291687	-	2229	WP_258707912.1	Ig-like domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-88)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10875.66 Da
• Isoelectric Point: 10.5014

>T225054 WP_252879826.1 NZ_CP087186:c2286971-2286687 [Pseudomonas siliginis]
MQIKWRPKARVELSKILKYIGERNFEAATALHKSVVKATSALCWHPHLYRKGRSPGTREIVVSPNYLVVYQVADHIEIVS
ILHARQEYPRRDPG

Nucleotide

• Length: 285 bp

>T225054 NZ_CP120944:352584-353111 [Escherichia coli O157:H7 str. EDL933]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAACGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7690.57 Da
• Isoelectric Point: 3.8068

>AT225054 WP_123463680.1 NZ_CP087186:c2287167-2286961 [Pseudomonas siliginis]
MIDPSLIDPLCPVVSDLDPEEQAASYDRWFRAEVQASLDDPRPSIPHEQVMEEMNALMESMRKSADAD

Nucleotide

• Length: 207 bp

>AT225054 NZ_CP120944:352311-352577 [Escherichia coli O157:H7 str. EDL933]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA