Detailed information of TA system TA225053

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1769060..1769676
• Replicon: chromosome
• Accession: NZ_CP087186
• Organism: Pseudomonas siliginis strain B21-063

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LOY48_RS07670
• Protein ID: WP_041479616.1
• Coordinates: 1769060..1769272 (+)
• Length: 71 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V8RA99
• Locus tag: LOY48_RS07675
• Protein ID: WP_016771035.1
• Coordinates: 1769272..1769676 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY48_RS07640 (LOY48_07625)	1765164..1765700	+	537	WP_024012128.1	DsbE family thiol:disulfide interchange protein	-
LOY48_RS07645 (LOY48_07630)	1765697..1766167	+	471	WP_258707790.1	cytochrome c-type biogenesis protein CcmH	-
LOY48_RS07650 (LOY48_07635)	1766164..1767363	+	1200	WP_252885574.1	c-type cytochrome biogenesis protein CcmI	-
LOY48_RS07655 (LOY48_07640)	1767389..1767793	+	405	WP_016771039.1	hypothetical protein	-
LOY48_RS07660 (LOY48_07645)	1767840..1768414	-	575	Protein_1507	PIN domain-containing protein	-
LOY48_RS07665 (LOY48_07650)	1768411..1768878	-	468	WP_252885575.1	helix-turn-helix domain-containing protein	-
LOY48_RS07670 (LOY48_07655)	1769060..1769272	+	213	WP_041479616.1	type II toxin-antitoxin system HicA family toxin	Toxin
LOY48_RS07675 (LOY48_07660)	1769272..1769676	+	405	WP_016771035.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LOY48_RS07680 (LOY48_07665)	1769789..1770988	-	1200	WP_252885576.1	MFS transporter	-
LOY48_RS07685 (LOY48_07670)	1771155..1772153	-	999	WP_056783861.1	sulfate ABC transporter substrate-binding protein	-
LOY48_RS07690 (LOY48_07675)	1772257..1773081	-	825	WP_041479613.1	ion transporter	-
LOY48_RS07695 (LOY48_07680)	1773103..1774017	-	915	WP_252885577.1	urea transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7960.12 Da
• Isoelectric Point: 10.5523

>T225053 WP_041479616.1 NZ_CP087186:1769060-1769272 [Pseudomonas siliginis]
VQSRLLMKELEEAGWTLDRVAGSHYIFTHRYNPNTIAVPHPKKDLPLGTVKSIRRRAGLFSPPTRHEGDP

Nucleotide

• Length: 213 bp

>T225053 NZ_CP120944:309880-310155 [Escherichia coli O157:H7 str. EDL933]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGC
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14776.81 Da
• Isoelectric Point: 4.5789

>AT225053 WP_016771035.1 NZ_CP087186:1769272-1769676 [Pseudomonas siliginis]
MQYPICIEWGDENTAIGIQIPDIPGAVTAGDSFEEAYNAAVEVAHLMLQEIAAAGQAIPMPTSAAAHRNHPDFAEMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHNVKSRSSFLADAAMEKLVRY

Nucleotide

• Length: 405 bp

>AT225053 NZ_CP120944:310152-310511 [Escherichia coli O157:H7 str. EDL933]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1N7DVA1