Detailed information of TA system TA225047

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1265224..1265852
• Replicon: chromosome
• Accession: NZ_CP087185
• Organism: Pseudomonas sichuanensis strain B21-027

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LOY51_RS05880
• Protein ID: WP_125857637.1
• Coordinates: 1265224..1265406 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LOY51_RS05885
• Protein ID: WP_125857638.1
• Coordinates: 1265427..1265852 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY51_RS05855 (LOY51_05855)	1261076..1262170	+	1095	WP_258645184.1	iron-sulfur cluster carrier protein ApbC	-
LOY51_RS05860 (LOY51_05860)	1262399..1262608	-	210	WP_016488765.1	cold-shock protein	-
LOY51_RS05865 (LOY51_05865)	1262949..1263515	+	567	WP_003258001.1	dCTP deaminase	-
LOY51_RS05870 (LOY51_05870)	1263614..1263844	+	231	WP_110995504.1	hypothetical protein	-
LOY51_RS05875 (LOY51_05875)	1263876..1264991	-	1116	WP_125857636.1	M14 family metallopeptidase	-
LOY51_RS05880 (LOY51_05880)	1265224..1265406	+	183	WP_125857637.1	type II toxin-antitoxin system HicA family toxin	Toxin
LOY51_RS05885 (LOY51_05885)	1265427..1265852	+	426	WP_125857638.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LOY51_RS05890 (LOY51_05890)	1265907..1268342	-	2436	WP_258645190.1	penicillin acylase family protein	-
LOY51_RS05895 (LOY51_05895)	1268531..1268734	-	204	WP_012313055.1	SEC-C metal-binding domain-containing protein	-
LOY51_RS05900 (LOY51_05900)	1268742..1269218	-	477	WP_258645193.1	LEA type 2 family protein	-
LOY51_RS05905 (LOY51_05905)	1269221..1269697	-	477	WP_125857641.1	YchJ family protein	-
LOY51_RS05910 (LOY51_05910)	1269702..1270196	-	495	WP_125462869.1	DUF6231 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-114)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6848.00 Da
• Isoelectric Point: 10.8431

>T225047 WP_125857637.1 NZ_CP087185:1265224-1265406 [Pseudomonas sichuanensis]
MKYSEFRRWLRAQGATFVPAKGSHFKVYLGKRQTIFPDHGAKEIGEGLRKKILKDLGLGD

Nucleotide

• Length: 183 bp

>T225047 NZ_CP120920:c1394198-1393452 [Bacillus paralicheniformis]
ATGCTGCTATTTTATCAATTCCTTGTTTGGCTCATTGTATTGGCGCTGGCTCTGTATGTGGCTGCCGTGTGGCGTTTTGA
AAAAAAGCTCACCGAAAAAACGGTCGCGATCCGAAAAACATGGTACCTGCTTTATGTTATCGGCGCCGTGATTTATTGGA
CGCACGATCCGCAGTCGATTTTCACAAACCCGCTTCATTACTTGATCGTTGCCGTTTTTTTCACGCTGACAGACGCTTTT
ATTTTCTTAAACGCCTACTTTAAAAAGCTCGGCAGCTCCGAACTCGCGACAGACACAAGAATGCTTCTCGAAGAAAACAA
CGACCTCCTCCACACATATCAAAACAGGCTGAAAACGTTTCAATACCTATTGAAAAACGAACCGATCCACATCTATTATG
GAAATATAGAAGCATATGCAGAAGGGATCGAAAAGCTCATCAAACGATTTGCCGAAAAAATGAATATATCCGCTGCACTT
TGCGAATATAATTCAGAAGAAAGCAAGGATCATTTGCTTGAGCATATGGAAAACCGCTTCGATGTTCAAGAAAAGCTTGA
GCGCAAAGACGTTTATTATGAAGAAAACGGAAAAATGGTCCTCATTCCTTTTTCCATCCACGATTTTGACTATGTCATGA
AGTTAACCTCAGAAGACCTTGTCACAGAATTTGACTACCTCCTGTTTACTTCTTTAACAAGCATCTATGATCTTCTGCTA
CCAAACGAAGAGGACGGTGATGACTGA

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15408.81 Da
• Isoelectric Point: 5.9738

>AT225047 WP_125857638.1 NZ_CP087185:1265427-1265852 [Pseudomonas sichuanensis]
MNFDYPVIVHCEAGSFWISCPDIPEMASAGGTMDEALFDAVDALESALSLYVDRRDAIPLPSPHGCDQAVVRLPALTAAK
AALWNTMIAQKVTKTELARRLGVNRPQVDRLVNLLHRSKIEQVEHALHVLGQRIELAVVAA

Nucleotide

• Length: 426 bp

>AT225047 NZ_CP120920:c1393452-1393279 [Bacillus paralicheniformis]
ATGGAGCCTGCTTTTCAGCAAGACCATGAAAGCGCAAAAAAGCGCCTCGCCTTTAAAATGTTTAAAAAGCAATCCAAAAC
CAATATCGCGAAATACGAAGTCAGCCCCCATACCGAACGGATTTTCCGGCAAAACGAACGGCTGATCGGCGAGTATAAAA
GAAAAAAAGCTTAA