Detailed information of TA system TA225043

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 5735019..5735541
• Replicon: chromosome
• Accession: NZ_CP087184
• Organism: Pseudomonas sp. B21-028

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LOY35_RS24880
• Protein ID: WP_258628371.1
• Coordinates: 5735248..5735541 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A6I6QD85
• Locus tag: LOY35_RS24875
• Protein ID: WP_047702740.1
• Coordinates: 5735019..5735258 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY35_RS24850 (LOY35_24815)	5730504..5731841	+	1338	WP_258628361.1	phosphatase PAP2/dual specificity phosphatase family protein	-
LOY35_RS24855 (LOY35_24820)	5731831..5732286	+	456	WP_258628363.1	hypothetical protein	-
LOY35_RS24860 (LOY35_24825)	5732296..5732916	+	621	WP_258628365.1	lysophospholipid acyltransferase family protein	-
LOY35_RS24865 (LOY35_24830)	5732916..5733848	+	933	WP_258628366.1	phosphatidate cytidylyltransferase	-
LOY35_RS24870 (LOY35_24835)	5733910..5734800	-	891	WP_258628367.1	MBL fold metallo-hydrolase	-
LOY35_RS24875 (LOY35_24840)	5735019..5735258	+	240	WP_047702740.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LOY35_RS24880 (LOY35_24845)	5735248..5735541	+	294	WP_258628371.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOY35_RS24885 (LOY35_24850)	5735598..5736449	-	852	WP_258628378.1	DUF2135 domain-containing protein	-
LOY35_RS24890 (LOY35_24855)	5736453..5738072	-	1620	WP_258628380.1	DUF2300 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-81)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11576.31 Da
• Isoelectric Point: 9.4064

>T225043 WP_258628371.1 NZ_CP087184:5735248-5735541 [Pseudomonas sp. B21-028]
MTYSLEFDRRALKEWNKLGDTLRQQFKNKLLEVLENPRIEANRLRQLPDCYKLKLRSAGYRLIYQVIDQEVVVFVVAIGK
REREAVYQDAQERISSD

Nucleotide

• Length: 294 bp

>T225043 NZ_CP120848:88727-89143 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8896.21 Da
• Isoelectric Point: 4.6017

>AT225043 WP_047702740.1 NZ_CP087184:5735019-5735258 [Pseudomonas sp. B21-028]
MASINIRIDDDLKQRSFAELEKLGVTPSELLRQTLQYVAERGKLPFKAALLSEEDEALIAVVTERLSNPQRVKVSLDDL

Nucleotide

• Length: 240 bp

>AT225043 NZ_CP120848:88500-88730 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A6I6QD85