Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 5512249..5512880 Replicon chromosome
Accession NZ_CP087183
Organism Pseudomonas putida strain B21-029

Toxin (Protein)


Gene name hicA Uniprot ID -
Locus tag LOY24_RS24745 Protein ID WP_258654026.1
Coordinates 5512653..5512880 (-) Length 76 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag LOY24_RS24740 Protein ID WP_258654025.1
Coordinates 5512249..5512656 (-) Length 136 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOY24_RS24720 (LOY24_24695) 5508782..5510233 + 1452 WP_258654023.1 phosphatase PAP2/dual specificity phosphatase family protein -
LOY24_RS24725 (LOY24_24700) 5510230..5510661 + 432 WP_258654024.1 hypothetical protein -
LOY24_RS24730 (LOY24_24705) 5510672..5511295 + 624 WP_016497906.1 lysophospholipid acyltransferase family protein -
LOY24_RS24735 (LOY24_24710) 5511297..5512229 + 933 WP_016489124.1 phosphatidate cytidylyltransferase -
LOY24_RS24740 (LOY24_24715) 5512249..5512656 - 408 WP_258654025.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
LOY24_RS24745 (LOY24_24720) 5512653..5512880 - 228 WP_258654026.1 type II toxin-antitoxin system HicA family toxin Toxin
LOY24_RS24750 (LOY24_24725) 5513233..5513576 + 344 Protein_4856 cupin domain-containing protein -
LOY24_RS24755 (LOY24_24730) 5513786..5514673 - 888 WP_019473211.1 3-hydroxyisobutyrate dehydrogenase -
LOY24_RS24760 (LOY24_24735) 5514683..5516209 - 1527 WP_258654027.1 CoA-acylating methylmalonate-semialdehyde dehydrogenase -
LOY24_RS24765 (LOY24_24740) 5516342..5517292 + 951 WP_258654029.1 LysR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-127)


Sequences

Toxin        


Download         Length: 76 a.a.        Molecular weight: 8217.32 Da        Isoelectric Point: 10.8172

>T225042 WP_258654026.1 NZ_CP087183:c5512880-5512653 [Pseudomonas putida]
MRSREVIQLIEADGWYEVEVKGSHHQFRHPTKKGRVTGPHPKTELPKGTVRSILKQAGLIQTGSVASTSVNGGNQ

Download         Length: 228 bp

>T225042 NZ_CP120848:c87692-87387 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 14683.57 Da        Isoelectric Point: 4.7269

>AT225042 WP_258654025.1 NZ_CP087183:c5512656-5512249 [Pseudomonas putida]
MKIPVVLHKDTCSDYGVTVPDVPGCFSAGATVAQALENVQEALALHFEGLVADNEPLPQAQEVDVHVANPDYEGGVWAVV
DFDVTPYLGKAVRFNATLPENLLQRIDEKVKRDHRYASRSGFLASAALRELALSH

Download         Length: 408 bp

>AT225042 NZ_CP120848:c87912-87694 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References