Detailed information of TA system TA225041

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 4854458..4856139
• Replicon: chromosome
• Accession: NZ_CP087183
• Organism: Pseudomonas putida strain B21-029

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: LOY24_RS21815
• Protein ID: WP_258653646.1
• Coordinates: 4854458..4855315 (+)
• Length: 286 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: LOY24_RS21820
• Protein ID: WP_026034667.1
• Coordinates: 4855321..4856139 (+)
• Length: 273 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY24_RS21790 (LOY24_21765)	4850456..4851286	+	831	WP_258657754.1	ABC transporter ATP-binding protein	-
LOY24_RS21795 (LOY24_21770)	4851318..4852328	+	1011	WP_258653640.1	ABC transporter substrate-binding protein	-
LOY24_RS21800 (LOY24_21775)	4852358..4853134	+	777	WP_258653641.1	ABC transporter permease	-
LOY24_RS21805 (LOY24_21780)	4853131..4853898	+	768	WP_258653643.1	SDR family oxidoreductase	-
LOY24_RS21810 (LOY24_21785)	4853914..4854453	+	540	WP_258653644.1	cupin domain-containing protein	-
LOY24_RS21815 (LOY24_21790)	4854458..4855315	+	858	WP_258653646.1	alpha/beta fold hydrolase	Toxin
LOY24_RS21820 (LOY24_21795)	4855321..4856139	+	819	WP_026034667.1	IclR family transcriptional regulator	Antitoxin
LOY24_RS21825 (LOY24_21800)	4856141..4856938	+	798	WP_258653647.1	SDR family oxidoreductase	-
LOY24_RS21830 (LOY24_21805)	4856931..4858613	+	1683	WP_258653648.1	thiamine pyrophosphate-binding protein	-
LOY24_RS21835 (LOY24_21810)	4858624..4859427	+	804	WP_258653650.1	aspartate dehydrogenase	-
LOY24_RS21840 (LOY24_21815)	4859438..4860928	+	1491	WP_258653651.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-268)

Antitoxin

• Transcription regulator IclR, C-terminal (131-253)

Sequences

Toxin

Protein

• Length: 286 a.a.
• Molecular weight: 30189.88 Da
• Isoelectric Point: 7.7706

>T225041 WP_258653646.1 NZ_CP087183:4854458-4855315 [Pseudomonas putida]
MMPITPIAPLLGQLDRRFAERLLRLEHGQQAVREAGSGPAMVLLHGIGSGAASWLQVALGLSERARVIAWDAPGYGPSTP
LPMAAPNARDYAARLLQTLDALAIERCVLVGHSLGALTAAAFAQAHPARVSRLVLLSPARGYGEQPEQGQAVRSKRLHTL
EHMGIEQMARERSAHLLSASASTQALAWVHWNMARLLPHGYRQAIELLCGDHLLRFAPPTVPCEVHCGSADAITPARDCQ
ALAEALGAPFKLIPGAGHACAIEQPDGVTGLLARALDTSLTGSVL

Nucleotide

• Length: 858 bp

>T225041 NZ_CP120848:61027-61443 [Klebsiella pneumoniae]
ATGACCAAAACCTACATGCTGGATACTAATATCTGCTCTTATATCATGCGCGAACAACCGGAAGCGGTGCTGAAACGCCT
AGAGCAAGCGGTGTTGCGCCGTCACCGGATAGTGGTGTCAGCCATCACCTATGCCGAAATGCGTTTTGGTGCCATCGGTA
AAAGGGCTTCGCCCCGTCATGTGCAGCTGGTGGACGCATTCTGTGCGCGCCTTGATGCCATACTGGCTTGGGATCGGGCC
GCAGTTGACGCTACAACAGAGGTTAAGGCCACGCTAGCAGCTGCCGGCACGCCAATCGGCCCAAACGACACAGCCATTGC
TGGACATGCCATTGCCGCTGGCGCCATCCTGGTGACGAATAATACGCGTGAATTTGAGCGAGTCGCTGGTCTGACGTTTG
AAGACTGGGTGCAATAA

Antitoxin

Protein

• Length: 273 a.a.
• Molecular weight: 30009.55 Da
• Isoelectric Point: 6.6424

>AT225041 WP_026034667.1 NZ_CP087183:4855321-4856139 [Pseudomonas putida]
MDPLKENQDKYIVPGLERGLLLLCEFSRQNRTLTAPELARRLSLPRSTIFRLLTTLETMGFVTRSGNEYRLGMSVLRLGF
EYLASLELTELGQPLLARLCDHLNYPSNLVVRDGRSIVYVAKVSPPSVFSSAVTVGTRLPAHATVLGRILLADLSLGELR
ELYPEEHLEQFSACTPKTVLELFDLVQADRLRGYVSGEGFFEPSISTVAAPVRDVSGQVVAALGLTIPTVQIGHVNFDNM
LGQVRNSADELSRLLNYTPNAGHGRVTALMRD

Nucleotide

• Length: 819 bp

>AT225041 NZ_CP120848:60800-61030 [Klebsiella pneumoniae]
ATGCGAACTGTATCTGTATTCAGGAACGGTAATAACCGGGCCATCCGTCTGCCCCGCGATCTGGACTTTGACGGCGTGAA
CGAACTGGAAATCATCCGCGAGGGCGATACCCTCATCCTGCGGCCGGTAAAACCCAGTTGGAGTTCGTTCCGCCAGGAGG
AAAAAGCTGACGCAGATTTTATGGCGGAGCGTGAAAATGTTGTTAGTGACGAGGGACGTTTTGAGCTATGA