Detailed information of TA system TA225038

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 3927712..3928595
• Replicon: chromosome
• Accession: NZ_CP087183
• Organism: Pseudomonas putida strain B21-029

Toxin (Protein)

• Gene name: -
• Uniprot ID: R9VG37
• Locus tag: LOY24_RS17350
• Protein ID: WP_016487683.1
• Coordinates: 3927712..3928149 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOY24_RS17355
• Protein ID: WP_258652739.1
• Coordinates: 3928146..3928595 (-)
• Length: 150 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY24_RS17335 (LOY24_17320)	3924060..3925289	-	1230	WP_028613239.1	acyl-CoA dehydrogenase	-
LOY24_RS17340 (LOY24_17325)	3925429..3926355	+	927	WP_063426843.1	LysR family transcriptional regulator	-
LOY24_RS17345 (LOY24_17330)	3926550..3927704	+	1155	WP_258652737.1	aminotransferase class V-fold PLP-dependent enzyme	-
LOY24_RS17350 (LOY24_17335)	3927712..3928149	-	438	WP_016487683.1	RES family NAD+ phosphorylase	Toxin
LOY24_RS17355 (LOY24_17340)	3928146..3928595	-	450	WP_258652739.1	MbcA/ParS/Xre antitoxin family protein	Antitoxin
LOY24_RS17360 (LOY24_17345)	3928702..3929478	-	777	WP_258652741.1	SDR family oxidoreductase	-
LOY24_RS17365 (LOY24_17350)	3929649..3930569	-	921	WP_258652743.1	LysR family transcriptional regulator	-
LOY24_RS17370 (LOY24_17355)	3930777..3931499	-	723	WP_258652745.1	hypothetical protein	-
LOY24_RS17375 (LOY24_17360)	3931579..3931878	-	300	WP_258652747.1	DUF3077 domain-containing protein	-
LOY24_RS17380 (LOY24_17365)	3932077..3932700	+	624	WP_258652749.1	glutathione S-transferase	-
LOY24_RS17385 (LOY24_17370)	3932782..3933288	+	507	WP_028613247.1	sigma-70 family RNA polymerase sigma factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-138)

Antitoxin

• Antitoxin Xre (29-146)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 16005.35 Da
• Isoelectric Point: 6.2187

>T225038 WP_016487683.1 NZ_CP087183:c3928149-3927712 [Pseudomonas putida]
VILWRISAYADLSGTGGLRVSGRWHQAGRPVVYAATSPSGAMLEVLVHLEIDPEDFPTTMRLIRIELPDTVSQAQLPALQ
PGWSAQPELTRALGNRFLDDCSALLLPVPSAIMPSTINYLFNPRHAQAQRAKIQVEDFTPDSRLF

Nucleotide

• Length: 438 bp

>T225038 NZ_CP120847:c5029638-5028721 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCATCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 150 a.a.
• Molecular weight: 17097.63 Da
• Isoelectric Point: 5.6804

>AT225038 WP_258652739.1 NZ_CP087183:c3928595-3928146 [Pseudomonas putida]
MLAEVLRDNGYHEYRARLEALLEIPELASDFEIHTRITHGFAATWLVKLTERGVFTPVERDQIIPLRTLKTRIERDQPLT
VEESDRLFRSAHITAMAEAVFGEADKAKRWLSKPKERFSGLTPMQMLTTQQGTTQVEEMLLQIAEGFGL

Nucleotide

• Length: 450 bp

>AT225038 NZ_CP120847:c5030402-5029644 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Toxin

• AlphaFold DB: R9VG37