Detailed information of TA system TA225037

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 2258608..2259124
• Replicon: chromosome
• Accession: NZ_CP087183
• Organism: Pseudomonas putida strain B21-029

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LOY24_RS10160
• Protein ID: WP_258656413.1
• Coordinates: 2258843..2259124 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1X0ZEN3
• Locus tag: LOY24_RS10155
• Protein ID: WP_016500420.1
• Coordinates: 2258608..2258853 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY24_RS10130 (LOY24_10125)	2254117..2255040	-	924	WP_258656404.1	LysR family transcriptional regulator	-
LOY24_RS10135 (LOY24_10130)	2255145..2255798	+	654	WP_132839664.1	oxygen-insensitive NAD(P)H nitroreductase	-
LOY24_RS10140 (LOY24_10135)	2255851..2256786	-	936	WP_258656407.1	cyclase family protein	-
LOY24_RS10145 (LOY24_10140)	2256883..2257425	-	543	WP_258656409.1	WYL domain-containing protein	-
LOY24_RS10150 (LOY24_10145)	2257543..2258340	-	798	WP_258656410.1	SDR family oxidoreductase	-
LOY24_RS10155 (LOY24_10150)	2258608..2258853	+	246	WP_016500420.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LOY24_RS10160 (LOY24_10155)	2258843..2259124	+	282	WP_258656413.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOY24_RS10165 (LOY24_10160)	2259144..2259776	-	633	WP_132839671.1	BRCT domain-containing protein	-
LOY24_RS10170 (LOY24_10165)	2259793..2260188	-	396	WP_061202459.1	hypothetical protein	-
LOY24_RS10175 (LOY24_10170)	2260309..2261217	-	909	WP_049275512.1	LysR family transcriptional regulator	-
LOY24_RS10180 (LOY24_10175)	2261447..2262751	+	1305	WP_258656417.1	MFS transporter	-
LOY24_RS10185 (LOY24_10180)	2262783..2264012	+	1230	WP_258656419.1	Zn-dependent hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-76)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10880.70 Da
• Isoelectric Point: 10.5787

>T225037 WP_258656413.1 NZ_CP087183:2258843-2259124 [Pseudomonas putida]
MTYKLEFLPSALKEWGKLGHTVREQIKKELGERLQSPKVQADALRDLPNHYKIKLKASGYRLVYRVEDERIVVVVVSVGK
RERSEVYRAAQKR

Nucleotide

• Length: 282 bp

>T225037 NZ_CP120847:c4873309-4873025 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8809.23 Da
• Isoelectric Point: 4.3611

>AT225037 WP_016500420.1 NZ_CP087183:2258608-2258853 [Pseudomonas putida]
MQNIFADVTVSVSELKKNPSAVMTGAAGMPVAVLNHNRVMGYMVPAELYEAMMERLEDLELSELAKSRLGEKGVPVSLDD
L

Nucleotide

• Length: 246 bp

>AT225037 NZ_CP120847:c4873541-4873299 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A1X0ZEN3